Proteomic analysis of cancer stem cells in human prostate cancer cells

Eun Kyung Lee, Hyungdon Cho, Chan Wha Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Results from recent studies support the hypothesis that cancer stem cells (CSCs) are responsible for tumor initiation and formation. Here, we applied a proteome profiling approach to investigate the mechanisms of CSCs and to identify potential biomarkers in the prostate cancer cell line DU145. Using MACS, the DU145 prostate cancer cell line was isolated into CD44+ or CD44- cells. In sphere culture, CD44+ cells possessed stem cell characteristics and highly expressed genes known to be important in stem cell maintenance. In addition, they showed strong tumorigenic potential in the clonogenic assay and soft agar colony formation assay. We then analyzed and identified proteins that were differentially expressed between CD44+ and CD44- using two-dimensional gel electrophoresis and LC-MS/MS. Cofilin and Annexin A5, which are associated with proliferation or metastasis in cancer, were found to be positively correlated with CD44 expression. These results provide information that will be important to the development of new cancer diagnostic tools and understanding the mechanisms of CSCs although a more detailed study is necessary to investigate the roles of Cofilin and Annexin A5 in CSCs.

Original languageEnglish
Pages (from-to)279-285
Number of pages7
JournalBiochemical and biophysical research communications
Volume412
Issue number2
DOIs
Publication statusPublished - 2011 Aug 26

Keywords

  • 2-Dimensional electrophoresis
  • CD44
  • Cancer stem cell
  • Prostate cancer

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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