Abstract
Hyperglycemia is a major key factor in the pathogenesis of microvascular complications of diabetes, including diabetic nephropathy (DN). Most studies to date have focused on the glomerular abnormalities found in DN. However, nephromegaly in the early stages of diabetes and the correlation of tubulointerstitial pathology rather than glomerular pathology with declining renal function in DN suggests the involvement of the tubulointerstitium. The etiology of the tubulointerstitial pathology in DN, however, is not fully understood. In this study, to understand the DN pathways, we constructed an initial 2-DE reference map for primitively cultured human proximal tubule (HK-2) cell in the presence of 5 mM and 25 mM glucose, which correspond to blood glucose concentrations during the normal and hyperglycemia conditions, respectively. Differentially expressed HK-2 cell cellular proteins at the high glucose concentration were identified via ESI-Q-TOF MS/MS and confirmed by Western blotting; enolase 1 (up-regulated) and lactate dehydrogenase (down-regulated). The regulation of these proteins will help in understanding DN mechanism through the glycolysis metabolic pathways in high glucose stimulated HK-2 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1118-1126 |
| Number of pages | 9 |
| Journal | Proteomics - Clinical Applications |
| Volume | 2 |
| Issue number | 7-8 |
| DOIs | |
| Publication status | Published - 2008 Jul 1 |
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Keywords
- 2-DE
- Diabetic nephropathy
- HK-2 cells
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Proteomic analysis of human proximal tubular cells exposed to high glucose concentrations. / So, Eun Jeong; Kim, Hyun Jung; Kim, Chan Wha.
In: Proteomics - Clinical Applications, Vol. 2, No. 7-8, 01.07.2008, p. 1118-1126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteomic analysis of human proximal tubular cells exposed to high glucose concentrations
AU - So, Eun Jeong
AU - Kim, Hyun Jung
AU - Kim, Chan Wha
PY - 2008/7/1
Y1 - 2008/7/1
N2 - Hyperglycemia is a major key factor in the pathogenesis of microvascular complications of diabetes, including diabetic nephropathy (DN). Most studies to date have focused on the glomerular abnormalities found in DN. However, nephromegaly in the early stages of diabetes and the correlation of tubulointerstitial pathology rather than glomerular pathology with declining renal function in DN suggests the involvement of the tubulointerstitium. The etiology of the tubulointerstitial pathology in DN, however, is not fully understood. In this study, to understand the DN pathways, we constructed an initial 2-DE reference map for primitively cultured human proximal tubule (HK-2) cell in the presence of 5 mM and 25 mM glucose, which correspond to blood glucose concentrations during the normal and hyperglycemia conditions, respectively. Differentially expressed HK-2 cell cellular proteins at the high glucose concentration were identified via ESI-Q-TOF MS/MS and confirmed by Western blotting; enolase 1 (up-regulated) and lactate dehydrogenase (down-regulated). The regulation of these proteins will help in understanding DN mechanism through the glycolysis metabolic pathways in high glucose stimulated HK-2 cells.
AB - Hyperglycemia is a major key factor in the pathogenesis of microvascular complications of diabetes, including diabetic nephropathy (DN). Most studies to date have focused on the glomerular abnormalities found in DN. However, nephromegaly in the early stages of diabetes and the correlation of tubulointerstitial pathology rather than glomerular pathology with declining renal function in DN suggests the involvement of the tubulointerstitium. The etiology of the tubulointerstitial pathology in DN, however, is not fully understood. In this study, to understand the DN pathways, we constructed an initial 2-DE reference map for primitively cultured human proximal tubule (HK-2) cell in the presence of 5 mM and 25 mM glucose, which correspond to blood glucose concentrations during the normal and hyperglycemia conditions, respectively. Differentially expressed HK-2 cell cellular proteins at the high glucose concentration were identified via ESI-Q-TOF MS/MS and confirmed by Western blotting; enolase 1 (up-regulated) and lactate dehydrogenase (down-regulated). The regulation of these proteins will help in understanding DN mechanism through the glycolysis metabolic pathways in high glucose stimulated HK-2 cells.
KW - 2-DE
KW - Diabetic nephropathy
KW - HK-2 cells
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UR - http://www.scopus.com/inward/citedby.url?scp=50849103366&partnerID=8YFLogxK
U2 - 10.1002/prca.200780151
DO - 10.1002/prca.200780151
M3 - Article
C2 - 21136909
AN - SCOPUS:50849103366
VL - 2
SP - 1118
EP - 1126
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
SN - 1862-8346
IS - 7-8
ER -