Proteomic analysis of human proximal tubular cells exposed to high glucose concentrations

Eun Jeong So, Hyun Jung Kim, Chan Wha Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hyperglycemia is a major key factor in the pathogenesis of microvascular complications of diabetes, including diabetic nephropathy (DN). Most studies to date have focused on the glomerular abnormalities found in DN. However, nephromegaly in the early stages of diabetes and the correlation of tubulointerstitial pathology rather than glomerular pathology with declining renal function in DN suggests the involvement of the tubulointerstitium. The etiology of the tubulointerstitial pathology in DN, however, is not fully understood. In this study, to understand the DN pathways, we constructed an initial 2-DE reference map for primitively cultured human proximal tubule (HK-2) cell in the presence of 5 mM and 25 mM glucose, which correspond to blood glucose concentrations during the normal and hyperglycemia conditions, respectively. Differentially expressed HK-2 cell cellular proteins at the high glucose concentration were identified via ESI-Q-TOF MS/MS and confirmed by Western blotting; enolase 1 (up-regulated) and lactate dehydrogenase (down-regulated). The regulation of these proteins will help in understanding DN mechanism through the glycolysis metabolic pathways in high glucose stimulated HK-2 cells.

Original languageEnglish
Pages (from-to)1118-1126
Number of pages9
JournalProteomics - Clinical Applications
Volume2
Issue number7-8
DOIs
Publication statusPublished - 2008 Jul

Keywords

  • 2-DE
  • Diabetic nephropathy
  • HK-2 cells

ASJC Scopus subject areas

  • Clinical Biochemistry

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