Proteomic analysis of mouse cortical neurons by isotope-coded affinity tags and mass spectrometry

Li Rong Yu, Mark D. Johnson, Thomas P. Conrads, Sang Won Lee, Ronald J. Moore, Nikola Tolic, Richard S. Morrison, Timothy D. Veenstra, Richard D. Smith

Research output: Contribution to conferencePaper

Abstract

The role of isotope coded affinity tags (ICAT) and mass spectrometry to measure protein expression changes between untreated and camptothecintreated mouse cortical neurons, was discussed. Equivalent amounts of protein extracted from the control and camptothecin-treated cortical neurons were labeled with the 'light' (ICAT-D 0) and the heavy (ICAT-D 8) isotopic version of the ICAT reagent. The sample was measured by cLC-Fourier transform ion cyclotron resonance mass spectrometry. More than 2,300 peptide pairs were detected in the FTICR-MS analysis.

Original languageEnglish
Pages281-282
Number of pages2
Publication statusPublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period02/6/202/6/6

ASJC Scopus subject areas

  • Spectroscopy

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  • Cite this

    Yu, L. R., Johnson, M. D., Conrads, T. P., Lee, S. W., Moore, R. J., Tolic, N., Morrison, R. S., Veenstra, T. D., & Smith, R. D. (2002). Proteomic analysis of mouse cortical neurons by isotope-coded affinity tags and mass spectrometry. 281-282. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.