Abstract
Objective: To construct a partial two-dimensional electrophoresis (2-DE) reference map of the proteins that compose normal human nasal mucosa. Design and methods: Normal inferior turbinate mucosa samples were subjected to 2-DE, the protein spots were visualized by silver staining, and 78 spots were selected for analysis by mass spectrometry and bioinformatics. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical techniques were performed for validation and localization analysis. Results: Among the identified proteins, the largest functional groups included proteins associated with the human immune response and enzymes, particularly those of protein metabolism. Proteins participating in the cell cycle, cell division, calcium metabolism, and ion transport were also detected. The mRNA transcripts for 10 selected proteins were amplified by RT-PCR. Immunohistochemistry revealed that secretagogin was localized in the submucosal gland and calsenilin was localized in the epithelium and submucosal gland. Conclusion: This database will serve as the basis for further comparative proteomic studies of nasal mucosal disorders.
| Original language | English |
|---|---|
| Pages (from-to) | 692-700 |
| Number of pages | 9 |
| Journal | Clinical Biochemistry |
| Volume | 42 |
| Issue number | 7-8 |
| DOIs | |
| Publication status | Published - 2009 May 1 |
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Keywords
- Human
- Immunohistochemistry
- Mass spectrometry
- Nasal mucosa
- Proteomics
- Reverse transcriptase polymerase chain reaction
- Two-dimensional electrophoresis
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Proteomic analysis of normal human nasal mucosa : Establishment of a two-dimensional electrophoresis reference map. / Lee, Jae Yong; Byun, Jang Yul; Lee, Sang Hag.
In: Clinical Biochemistry, Vol. 42, No. 7-8, 01.05.2009, p. 692-700.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteomic analysis of normal human nasal mucosa
T2 - Establishment of a two-dimensional electrophoresis reference map
AU - Lee, Jae Yong
AU - Byun, Jang Yul
AU - Lee, Sang Hag
PY - 2009/5/1
Y1 - 2009/5/1
N2 - Objective: To construct a partial two-dimensional electrophoresis (2-DE) reference map of the proteins that compose normal human nasal mucosa. Design and methods: Normal inferior turbinate mucosa samples were subjected to 2-DE, the protein spots were visualized by silver staining, and 78 spots were selected for analysis by mass spectrometry and bioinformatics. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical techniques were performed for validation and localization analysis. Results: Among the identified proteins, the largest functional groups included proteins associated with the human immune response and enzymes, particularly those of protein metabolism. Proteins participating in the cell cycle, cell division, calcium metabolism, and ion transport were also detected. The mRNA transcripts for 10 selected proteins were amplified by RT-PCR. Immunohistochemistry revealed that secretagogin was localized in the submucosal gland and calsenilin was localized in the epithelium and submucosal gland. Conclusion: This database will serve as the basis for further comparative proteomic studies of nasal mucosal disorders.
AB - Objective: To construct a partial two-dimensional electrophoresis (2-DE) reference map of the proteins that compose normal human nasal mucosa. Design and methods: Normal inferior turbinate mucosa samples were subjected to 2-DE, the protein spots were visualized by silver staining, and 78 spots were selected for analysis by mass spectrometry and bioinformatics. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical techniques were performed for validation and localization analysis. Results: Among the identified proteins, the largest functional groups included proteins associated with the human immune response and enzymes, particularly those of protein metabolism. Proteins participating in the cell cycle, cell division, calcium metabolism, and ion transport were also detected. The mRNA transcripts for 10 selected proteins were amplified by RT-PCR. Immunohistochemistry revealed that secretagogin was localized in the submucosal gland and calsenilin was localized in the epithelium and submucosal gland. Conclusion: This database will serve as the basis for further comparative proteomic studies of nasal mucosal disorders.
KW - Human
KW - Immunohistochemistry
KW - Mass spectrometry
KW - Nasal mucosa
KW - Proteomics
KW - Reverse transcriptase polymerase chain reaction
KW - Two-dimensional electrophoresis
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UR - http://www.scopus.com/inward/citedby.url?scp=63649157664&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2008.12.022
DO - 10.1016/j.clinbiochem.2008.12.022
M3 - Article
C2 - 19167376
AN - SCOPUS:63649157664
VL - 42
SP - 692
EP - 700
JO - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 7-8
ER -