TY - JOUR
T1 - Proteomic analysis of plasma proteins of workers exposed to benzene
AU - Joo, Won A.
AU - Sul, Donggeun
AU - Lee, Do Youn
AU - Lee, Eunil
AU - Kim, Chan Wha
N1 - Funding Information:
This work was supported by the Korean Ministry of Commerce, Industry and Energy and by a grant from Korea University Grant. Won-A Joo hold a Brain Korea 21 fellowship from the Korean Ministry of Education and Human Resources Development. We express our gratitude to Dr. Sangduk Kim for helping with the manuscript preparation and for providing scientific discussion. We also appreciate the help of Jong-Bok Seo (Korea Basic Science Institute) for performing the MALDI-TOF MS analysis.
PY - 2004/3/14
Y1 - 2004/3/14
N2 - In this study, we analyzed the proteins in plasma of workers exposed to benzene by two-dimensional gel electrophoresis, in the hope of finding a specific protein suitable for the biomonitoring of benzene exposure. Comet assays were also carried out to evaluate lymphocytes DNA damage. Fifty workers from a printing company and 38 matched unexposed healthy subjects were enrolled in the study. DNA damage was found to be significantly higher in the exposed workers than in the controls. The tail moments of the two groups were 2.07±0.35 and 1.48±0.41, respectively (P<0.0001). The mean values of trans, trans-muconic acid (t,t-MA) in workers exposed to benzene and in unexposed subjects were 1.011±0.249 and 0.026±0.028mg/g creatinine, respectively. Protein profiles were significantly different (P<0.05) in the two groups, as identified by matrix-assisted laser desorption ionization/time of flight (MALDI-TOF) mass spectrometry and confirmed by Western blot. T cell receptor β chain (TCR β), FK506-binding protein (FKBP51) and matrix metalloproteinase-13 (MMP13) were found to be up-regulated in the benzene-exposed workers. In addition, the correlation between TCR β and the tail moments of lymphocytes was statistically significant (r-value, 0.428). We conclude that TCR β in plasma could be used for the early detection of exposure to benzene.
AB - In this study, we analyzed the proteins in plasma of workers exposed to benzene by two-dimensional gel electrophoresis, in the hope of finding a specific protein suitable for the biomonitoring of benzene exposure. Comet assays were also carried out to evaluate lymphocytes DNA damage. Fifty workers from a printing company and 38 matched unexposed healthy subjects were enrolled in the study. DNA damage was found to be significantly higher in the exposed workers than in the controls. The tail moments of the two groups were 2.07±0.35 and 1.48±0.41, respectively (P<0.0001). The mean values of trans, trans-muconic acid (t,t-MA) in workers exposed to benzene and in unexposed subjects were 1.011±0.249 and 0.026±0.028mg/g creatinine, respectively. Protein profiles were significantly different (P<0.05) in the two groups, as identified by matrix-assisted laser desorption ionization/time of flight (MALDI-TOF) mass spectrometry and confirmed by Western blot. T cell receptor β chain (TCR β), FK506-binding protein (FKBP51) and matrix metalloproteinase-13 (MMP13) were found to be up-regulated in the benzene-exposed workers. In addition, the correlation between TCR β and the tail moments of lymphocytes was statistically significant (r-value, 0.428). We conclude that TCR β in plasma could be used for the early detection of exposure to benzene.
KW - Benzene
KW - Comet assay
KW - MALDI-TOF
KW - TCR β
KW - Two-dimensional electrophoresis (2-DE)
UR - http://www.scopus.com/inward/record.url?scp=1242317101&partnerID=8YFLogxK
U2 - 10.1016/j.mrgentox.2003.10.015
DO - 10.1016/j.mrgentox.2003.10.015
M3 - Article
C2 - 15036117
AN - SCOPUS:1242317101
SN - 1383-5718
VL - 558
SP - 35
EP - 44
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
IS - 1-2
ER -