Proteomic analysis of plasma proteins of workers exposed to benzene

Won A. Joo, Dong Geun Sul, Do Youn Lee, Eun Il Lee, Chan Wha Kim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In this study, we analyzed the proteins in plasma of workers exposed to benzene by two-dimensional gel electrophoresis, in the hope of finding a specific protein suitable for the biomonitoring of benzene exposure. Comet assays were also carried out to evaluate lymphocytes DNA damage. Fifty workers from a printing company and 38 matched unexposed healthy subjects were enrolled in the study. DNA damage was found to be significantly higher in the exposed workers than in the controls. The tail moments of the two groups were 2.07±0.35 and 1.48±0.41, respectively (P<0.0001). The mean values of trans, trans-muconic acid (t,t-MA) in workers exposed to benzene and in unexposed subjects were 1.011±0.249 and 0.026±0.028mg/g creatinine, respectively. Protein profiles were significantly different (P<0.05) in the two groups, as identified by matrix-assisted laser desorption ionization/time of flight (MALDI-TOF) mass spectrometry and confirmed by Western blot. T cell receptor β chain (TCR β), FK506-binding protein (FKBP51) and matrix metalloproteinase-13 (MMP13) were found to be up-regulated in the benzene-exposed workers. In addition, the correlation between TCR β and the tail moments of lymphocytes was statistically significant (r-value, 0.428). We conclude that TCR β in plasma could be used for the early detection of exposure to benzene.

Original languageEnglish
Pages (from-to)35-44
Number of pages10
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume558
Issue number1-2
DOIs
Publication statusPublished - 2004 Mar 14

Fingerprint

Benzene
Proteomics
Blood Proteins
T-Cell Antigen Receptor
DNA Damage
Tacrolimus Binding Proteins
Lymphocytes
Matrix Metalloproteinase 13
Printing
Comet Assay
Environmental Monitoring
Electrophoresis, Gel, Two-Dimensional
Mass Spectrometry
Creatinine
Healthy Volunteers
Proteins
Lasers
Western Blotting

Keywords

  • Benzene
  • Comet assay
  • MALDI-TOF
  • TCR β
  • Two-dimensional electrophoresis (2-DE)

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

Proteomic analysis of plasma proteins of workers exposed to benzene. / Joo, Won A.; Sul, Dong Geun; Lee, Do Youn; Lee, Eun Il; Kim, Chan Wha.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 558, No. 1-2, 14.03.2004, p. 35-44.

Research output: Contribution to journalArticle

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