Proteomic analysis of proteins secreted by HepG2 cells treated with butyl benzyl phthalate

Seonyoung Choi, So Young Park, Dongsub Kwak, Sohee Phark, Min Lee, Ji Youn Lim, Woon Won Jung, Dong Geun Sul

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 μM) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate isomerase, heptaglobin-related protein, inter-alpha-trypsin inhibitor heavy chain H2, and electron transfer flavoprotein subunit beta were confirmed by Western blot analysis. These proteins were found to be involved in apoptosis, signaling, tumor progression, energy metabolism, and cell structure and motility. Therefore, these proteins have potential to be employed as biomarkers of BBP exposure and may be useful in understanding mechanisms underlying the adverse effects of BBP.

Original languageEnglish
Pages (from-to)1570-1585
Number of pages16
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume73
Issue number21-22
DOIs
Publication statusPublished - 2010 Oct 28

Fingerprint

Hep G2 Cells
Proteomics
Proteins
rho-Specific Guanine Nucleotide Dissociation Inhibitors
Electron-Transferring Flavoproteins
Mass spectrometry
raf Kinases
Gelsolin
Triose-Phosphate Isomerase
Cystatin C
Electrospray Ionization Mass Spectrometry
Electrospray ionization
Electrophoresis, Gel, Two-Dimensional
Tandem Mass Spectrometry
butylbenzyl phthalate
Biomarkers
Protein Kinases
Energy Metabolism
Cell Movement
Electrophoresis

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Proteomic analysis of proteins secreted by HepG2 cells treated with butyl benzyl phthalate. / Choi, Seonyoung; Park, So Young; Kwak, Dongsub; Phark, Sohee; Lee, Min; Lim, Ji Youn; Jung, Woon Won; Sul, Dong Geun.

In: Journal of Toxicology and Environmental Health - Part A: Current Issues, Vol. 73, No. 21-22, 28.10.2010, p. 1570-1585.

Research output: Contribution to journalArticle

Choi, Seonyoung ; Park, So Young ; Kwak, Dongsub ; Phark, Sohee ; Lee, Min ; Lim, Ji Youn ; Jung, Woon Won ; Sul, Dong Geun. / Proteomic analysis of proteins secreted by HepG2 cells treated with butyl benzyl phthalate. In: Journal of Toxicology and Environmental Health - Part A: Current Issues. 2010 ; Vol. 73, No. 21-22. pp. 1570-1585.
@article{6ed7f82ef72242ae8bd7d8963457e69b,
title = "Proteomic analysis of proteins secreted by HepG2 cells treated with butyl benzyl phthalate",
abstract = "Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 μM) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate isomerase, heptaglobin-related protein, inter-alpha-trypsin inhibitor heavy chain H2, and electron transfer flavoprotein subunit beta were confirmed by Western blot analysis. These proteins were found to be involved in apoptosis, signaling, tumor progression, energy metabolism, and cell structure and motility. Therefore, these proteins have potential to be employed as biomarkers of BBP exposure and may be useful in understanding mechanisms underlying the adverse effects of BBP.",
author = "Seonyoung Choi and Park, {So Young} and Dongsub Kwak and Sohee Phark and Min Lee and Lim, {Ji Youn} and Jung, {Woon Won} and Sul, {Dong Geun}",
year = "2010",
month = "10",
day = "28",
doi = "10.1080/15287394.2010.511583",
language = "English",
volume = "73",
pages = "1570--1585",
journal = "Journal of Toxicology and Environmental Health - Part A: Current Issues",
issn = "1528-7394",
publisher = "Taylor and Francis Ltd.",
number = "21-22",

}

TY - JOUR

T1 - Proteomic analysis of proteins secreted by HepG2 cells treated with butyl benzyl phthalate

AU - Choi, Seonyoung

AU - Park, So Young

AU - Kwak, Dongsub

AU - Phark, Sohee

AU - Lee, Min

AU - Lim, Ji Youn

AU - Jung, Woon Won

AU - Sul, Dong Geun

PY - 2010/10/28

Y1 - 2010/10/28

N2 - Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 μM) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate isomerase, heptaglobin-related protein, inter-alpha-trypsin inhibitor heavy chain H2, and electron transfer flavoprotein subunit beta were confirmed by Western blot analysis. These proteins were found to be involved in apoptosis, signaling, tumor progression, energy metabolism, and cell structure and motility. Therefore, these proteins have potential to be employed as biomarkers of BBP exposure and may be useful in understanding mechanisms underlying the adverse effects of BBP.

AB - Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 μM) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate isomerase, heptaglobin-related protein, inter-alpha-trypsin inhibitor heavy chain H2, and electron transfer flavoprotein subunit beta were confirmed by Western blot analysis. These proteins were found to be involved in apoptosis, signaling, tumor progression, energy metabolism, and cell structure and motility. Therefore, these proteins have potential to be employed as biomarkers of BBP exposure and may be useful in understanding mechanisms underlying the adverse effects of BBP.

UR - http://www.scopus.com/inward/record.url?scp=77958171659&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958171659&partnerID=8YFLogxK

U2 - 10.1080/15287394.2010.511583

DO - 10.1080/15287394.2010.511583

M3 - Article

VL - 73

SP - 1570

EP - 1585

JO - Journal of Toxicology and Environmental Health - Part A: Current Issues

JF - Journal of Toxicology and Environmental Health - Part A: Current Issues

SN - 1528-7394

IS - 21-22

ER -