Proteomic analysis of pulmonary sclerosing hemangioma

Lian Jin Jin, Bong Kyung Shin, Woon Yong Jung, Hyun Juu Lee, Su Jin Cho, Joung Ho Han, Seong Yeon Ha, Ae Ree Kim, Young Sik Kim, In Sun Kim, Chang Sub Uhm, Han Kyeom Kim

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11 Citations (Scopus)

Abstract

Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been dear. Surfactant protein, thyroid transcription factor-1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2-D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI-TOF-MS, and database searching. Apolipoprotein A-1, antizyme inhibitor, heat shock 27-kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down-regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.

Original languageEnglish
Pages (from-to)4877-4883
Number of pages7
JournalProteomics
Volume6
Issue number17
DOIs
Publication statusPublished - 2006 Sep

Keywords

  • 2-D PAGE
  • GST
  • Prx II
  • Sclerosing hemangioma

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Jin, L. J., Shin, B. K., Jung, W. Y., Lee, H. J., Cho, S. J., Han, J. H., Ha, S. Y., Kim, A. R., Kim, Y. S., Kim, I. S., Uhm, C. S., & Kim, H. K. (2006). Proteomic analysis of pulmonary sclerosing hemangioma. Proteomics, 6(17), 4877-4883. https://doi.org/10.1002/pmic.200500819