Proteomic analysis of pulmonary sclerosing hemangioma

Lian Jin Jin, Bong Kyung Shin, Woon Yong Jung, Hyun Juu Lee, Su Jin Cho, Joung Ho Han, Seong Yeon Ha, Aeree Kim, Young Sik Kim, In Sun Kim, Chang Sub Uhm, Han Kyeom Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been dear. Surfactant protein, thyroid transcription factor-1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2-D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI-TOF-MS, and database searching. Apolipoprotein A-1, antizyme inhibitor, heat shock 27-kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down-regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.

Original languageEnglish
Pages (from-to)4877-4883
Number of pages7
JournalProteomics
Volume6
Issue number17
DOIs
Publication statusPublished - 2006 Sep 1

Fingerprint

Pulmonary Sclerosing Hemangioma
Benign Fibrous Histiocytoma
Proteomics
Peroxiredoxins
Proteins
Tissue
Mucin-1
Immunohistochemistry
Apolipoprotein A-I
Vimentin
HSP27 Heat-Shock Proteins
Keratins
Surface-Active Agents
Respiratory Mucosa
Lung
Electron microscopy
Tumors
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Antioxidants
Electron Microscopy

Keywords

  • 2-D PAGE
  • GST
  • Prx II
  • Sclerosing hemangioma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Jin, L. J., Shin, B. K., Jung, W. Y., Lee, H. J., Cho, S. J., Han, J. H., ... Kim, H. K. (2006). Proteomic analysis of pulmonary sclerosing hemangioma. Proteomics, 6(17), 4877-4883. https://doi.org/10.1002/pmic.200500819

Proteomic analysis of pulmonary sclerosing hemangioma. / Jin, Lian Jin; Shin, Bong Kyung; Jung, Woon Yong; Lee, Hyun Juu; Cho, Su Jin; Han, Joung Ho; Ha, Seong Yeon; Kim, Aeree; Kim, Young Sik; Kim, In Sun; Uhm, Chang Sub; Kim, Han Kyeom.

In: Proteomics, Vol. 6, No. 17, 01.09.2006, p. 4877-4883.

Research output: Contribution to journalArticle

Jin, LJ, Shin, BK, Jung, WY, Lee, HJ, Cho, SJ, Han, JH, Ha, SY, Kim, A, Kim, YS, Kim, IS, Uhm, CS & Kim, HK 2006, 'Proteomic analysis of pulmonary sclerosing hemangioma', Proteomics, vol. 6, no. 17, pp. 4877-4883. https://doi.org/10.1002/pmic.200500819
Jin LJ, Shin BK, Jung WY, Lee HJ, Cho SJ, Han JH et al. Proteomic analysis of pulmonary sclerosing hemangioma. Proteomics. 2006 Sep 1;6(17):4877-4883. https://doi.org/10.1002/pmic.200500819
Jin, Lian Jin ; Shin, Bong Kyung ; Jung, Woon Yong ; Lee, Hyun Juu ; Cho, Su Jin ; Han, Joung Ho ; Ha, Seong Yeon ; Kim, Aeree ; Kim, Young Sik ; Kim, In Sun ; Uhm, Chang Sub ; Kim, Han Kyeom. / Proteomic analysis of pulmonary sclerosing hemangioma. In: Proteomics. 2006 ; Vol. 6, No. 17. pp. 4877-4883.
@article{ca95ca4849f54617a3acb2219b743329,
title = "Proteomic analysis of pulmonary sclerosing hemangioma",
abstract = "Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been dear. Surfactant protein, thyroid transcription factor-1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2-D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI-TOF-MS, and database searching. Apolipoprotein A-1, antizyme inhibitor, heat shock 27-kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down-regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.",
keywords = "2-D PAGE, GST, Prx II, Sclerosing hemangioma",
author = "Jin, {Lian Jin} and Shin, {Bong Kyung} and Jung, {Woon Yong} and Lee, {Hyun Juu} and Cho, {Su Jin} and Han, {Joung Ho} and Ha, {Seong Yeon} and Aeree Kim and Kim, {Young Sik} and Kim, {In Sun} and Uhm, {Chang Sub} and Kim, {Han Kyeom}",
year = "2006",
month = "9",
day = "1",
doi = "10.1002/pmic.200500819",
language = "English",
volume = "6",
pages = "4877--4883",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "17",

}

TY - JOUR

T1 - Proteomic analysis of pulmonary sclerosing hemangioma

AU - Jin, Lian Jin

AU - Shin, Bong Kyung

AU - Jung, Woon Yong

AU - Lee, Hyun Juu

AU - Cho, Su Jin

AU - Han, Joung Ho

AU - Ha, Seong Yeon

AU - Kim, Aeree

AU - Kim, Young Sik

AU - Kim, In Sun

AU - Uhm, Chang Sub

AU - Kim, Han Kyeom

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been dear. Surfactant protein, thyroid transcription factor-1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2-D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI-TOF-MS, and database searching. Apolipoprotein A-1, antizyme inhibitor, heat shock 27-kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down-regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.

AB - Sclerosing hemangioma (SH) is a rare benign pulmonary tumor derived from the primitive respiratory epithelium. However, the pathogenesis of SH has not yet been dear. Surfactant protein, thyroid transcription factor-1, epithelial membrane antigen, cytokeratin, and vimentin have been identified in SH by immunohistochemistry and electron microscopy. To identify proteins specifically regulated in SH, 2-D PAGE was performed using SH and paired normal tissues. Ten selected differentially expressed protein spots were identified by PMF, MALDI-TOF-MS, and database searching. Apolipoprotein A-1, antizyme inhibitor, heat shock 27-kDa protein 1, and antioxidant proteins, such as peroxiredoxin II (Prx II) and GST, were identified among the down-regulated proteins in SH. Western blot and immunohistochemistry confirmed reduced expressions of Prx II and GST in SH versus normal lung tissue. This study is the first report on the reduced expressions of Prx II and GST in SH.

KW - 2-D PAGE

KW - GST

KW - Prx II

KW - Sclerosing hemangioma

UR - http://www.scopus.com/inward/record.url?scp=33748572962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748572962&partnerID=8YFLogxK

U2 - 10.1002/pmic.200500819

DO - 10.1002/pmic.200500819

M3 - Article

VL - 6

SP - 4877

EP - 4883

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 17

ER -