Proteomic analysis of pure human airway gland mucus reveals a large component of protective proteins

Nam Soo Joo, Idil Apak T. Evans, Hyung Ju Cho, Il Ho Park, John F. Engelhardt, Jeffrey J. Wine

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Airway submucosal glands contribute to innate immunity and protect the lungs by secreting mucus, which is required for mucociliary clearance and which also contains antimicrobial, anti-inflammatory, anti-proteolytic and anti-oxidant proteins. We stimulated glands in tracheal trimmings from three lung donors and collected droplets of uncontaminated mucus as they formed at the gland orifices under an oil layer. We analyzed the mucus using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analysis identified 5486 peptides and 441 proteins from across the 3 samples (269-319 proteins per subject).We focused on 269 proteins common to at least 2 0f 3 subjects, of which 102 (38%) had protective or innate immunity functions. While many of these have long been known to play such roles, for many others their cellular protective functions have only recently been appreciated in addition to their well-studied biologic functions (e.g. annexins, apolipoproteins, gelsolin, hemoglobin, histones, keratins, and lumican). A minority of the identified proteins are known to be secreted via conventional exocytosis, suggesting that glandular secretion occurs via multiplemechanisms. Two of the observed protective proteins, major vault protein and prohibitin, have not been observed in fluid fromhuman epithelial cultures or in fluid fromnasal or bronchoalveolar lavage. Further proteomic analysis of pure gland mucus may help clarify how healthy airways maintain a sterile environment.

Original languageEnglish
Article numbere0116756
JournalPLoS One
Volume10
Issue number2
DOIs
Publication statusPublished - 2015 Feb 23

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Mucus
mucus
Proteomics
proteomics
Proteins
proteins
Innate Immunity
Gelsolin
Annexins
Fluids
Trimming
Apolipoproteins
Liquid chromatography
Mucociliary Clearance
lungs
Lung
Keratins
Orifices
Oxidants
Histones

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Proteomic analysis of pure human airway gland mucus reveals a large component of protective proteins. / Joo, Nam Soo; Evans, Idil Apak T.; Cho, Hyung Ju; Park, Il Ho; Engelhardt, John F.; Wine, Jeffrey J.

In: PLoS One, Vol. 10, No. 2, e0116756, 23.02.2015.

Research output: Contribution to journalArticle

Joo, Nam Soo ; Evans, Idil Apak T. ; Cho, Hyung Ju ; Park, Il Ho ; Engelhardt, John F. ; Wine, Jeffrey J. / Proteomic analysis of pure human airway gland mucus reveals a large component of protective proteins. In: PLoS One. 2015 ; Vol. 10, No. 2.
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