Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy

Pyong Gon Moon, Jeong Eun Lee, Sungyong You, Taek Kyun Kim, Ji Hoon Cho, In San Kim, Tae Hwan Kwon, Chan Duck Kim, Sun Hee Park, Daehee Hwang, Yong Lim Kim, Moon Chang Baek

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

To identify biomarker candidates associated with early IgA nephropathy (IgAN) and thin basement membrane nephropathy (TBMN), the most common causes presenting isolated hematuria in childhood, a proteomic approach of urinary exosomes from early IgAN and TBMN patients was introduced. The proteomic results from the patients were compared with a normal group to understand the pathophysiological processes associated with these diseases at the protein level. The urinary exosomes, which reflect pathophysiological processes, collected from three groups of young adults (early IgAN, TBMN, and normal) were trypsin-digested using a gel-assisted protocol, and quantified by label-free LC-MS/MS, using an MSE mode. A total of 1877 urinary exosome proteins, including cytoplasmic, membrane, and vesicle trafficking proteins, were identified. Among the differentially expressed proteins, four proteins (aminopeptidase N, vasorin precursor, α-1-antitrypsin, and ceruloplasmin) were selected as biomarker candidates to differentiate early IgAN from TBMN. We confirmed the protein levels of the four biomarker candidates by semi-quantitative immunoblot analysis in urinary exosomes independently prepared from other patients, including older adult groups. Further clinical studies are needed to investigate the diagnostic and prognostic value of these urinary markers for early IgAN and TBMN. Taken together, this study showed the possibility of identifying biomarker candidates for human urinary diseases using urinary exosomes and might help to understand the pathophysiology of early IgAN and TBMN at the protein level.

Original languageEnglish
Pages (from-to)2459-2475
Number of pages17
JournalProteomics
Volume11
Issue number12
DOIs
Publication statusPublished - 2011 Jun
Externally publishedYes

Keywords

  • Biomarker
  • Biomedicine
  • Exosomes
  • IgA nephropathy
  • Thin basement membrane nephropathy

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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