Proteomic characterization of rat liver exposed to 2,3,7,8- tetrachlorobenzo-p-dioxin

Sung Han Lee, Do Youn Lee, Won Kyu Son, Won A. Joo, Chan Wha Kim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Dioxins are a class of polyhalogenated aromatic hydrocarbons that induce a wide spectrum of toxic responses in experimental animals. In this study, 2,3,7,8-tetrachlorobenzo-p-dioxin (TCDD) was exposed to two SD rat groups; one group for short-term exposure at a single dose of 1, 10, 20 and 50 μg/kg body weight (group 1) and the other for long-term exposure at daily and-low dose of 0.01, 0.1, 1 and 2.5 μg/kg body weight (group 2) for a month. Two-dimensional electrophoresis (2-DE) was utilized to resolve the protein profile of rat liver exposed to TCDD at different doses. In the analysis of 2-DE of the group 1, two new-expressed spots and seven volume-increased spots were detected and identified by ESI-Q-TOF MS/MS; especially, proteasome subunit beta type 3 was increased in all doses. In addition, in the group 2, six volume-increased spots were screened; particularly, histidine triad nucleotide binding protein was increased in both 0.1 μg/kg dose and 1 μg/kg dose. The identified proteins were confirmed using Western blot. Among the identified proteins, apolipoprotein A-IV may protect lipid peroxidation and atherosclerosis induced by TCDD exposure and the expression level of phosphoglycerate mutase increases due to hyperthyroidism induced by TCDD exposure.

Original languageEnglish
Pages (from-to)335-343
Number of pages9
JournalJournal of Proteome Research
Volume4
Issue number2
DOIs
Publication statusPublished - 2005 Mar

Keywords

  • 2,3,7,8-tetrachlorobenzo-p-dioxin
  • Atherosclerosis hyperthyroidism
  • Rat liver
  • Thyroid hormone receptor α

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Proteomic characterization of rat liver exposed to 2,3,7,8- tetrachlorobenzo-p-dioxin'. Together they form a unique fingerprint.

Cite this