Proteomic comparison of Gibberella moniliformis in limited-nitrogen (fumonisin-inducing) and excess-nitrogen (fumonisin-repressing) conditions

Yoon-E Choi, Robert A.E. Butchko, Won Bo Shim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The maize pathogen Gibberella moniliformis produces fumonisins, a group of mycotoxins associated with several disorders in animals and humans, including cancer. The current focus of our research is to understand the regulatory mechanisms involved in fumonisin biosynthesis. In this study, we employed a proteomics approach to identify novel genes involved in the fumonisin biosynthesis under nitrogen stress. The combination of genome sequence, mutant strains, EST database, microarrays, and proteomics offers an opportunity to advance our understanding of this process. We investigated the response of the G moniliformis proteome in limited nitrogen (NO, fumonisin-inducing) and excess nitrogen (N+, fumonisin-repressing) conditions by one- and two-dimensional electrophoresis. We selected 11 differentially expressed proteins, six from limited nitrogen conditions and five from excess nitrogen conditions, and determined the sequences by peptide mass fingerprinting and MS/MS spectrophotometry. Subsequently, we identified the EST sequences corresponding to the proteins and studied their expression profiles in different culture conditions. Through the comparative analysis of gene and protein expression data, we identified three candidate genes for functional analysis and our results provided valuable clues regarding the regulatory mechanisms of fumonisin biosynthesis.

Original languageEnglish
Pages (from-to)780-787
Number of pages8
JournalJournal of microbiology and biotechnology
Volume22
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1
Externally publishedYes

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Keywords

  • Fumonisin regulation
  • Fusarium verticillioides
  • Gene expression
  • Nitrogen metabolite repression
  • Proteomics

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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