Proteomic evaluation of cadmium toxicity on the midge Chironomus riparius Meigen larvae

Sung Eun Lee, Dong Hun Yoo, Jino Son, Ki Jong Cho

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Heavy-metal pollution of aquatic ecosystems is a widespread phenomenon after industrial consumption. Whether aquatic organisms are adapted to the heavy-metal pollutants or not, such environmental stress causes changes in physiological responses. In this study, the aquatic midge, Chironomus riparius Meigen, was used to find changes of expression of proteins in relation to cadmium exposure. Dose-response relationships between cadmium concentrations and mortality of 3rd instar midge larvae were observed and the protein levels were compared using PD-Quest after 2-DE. Comparing the intensity of protein spots, 21 proteins decreased and 18 proteins increased in response to cadmium treatment. With increased proteins, three enzymes such as S-adenosylmethionine decarboxylase, O-methyltransferase, and aspartokinase were involved in the glutathione biosynthesis and a key enzyme regulating fatty acid biosynthesis, oleyl-acyl carrier protein thioesterase was also identified. According to the functional classification of decreased levels of proteins, they were involved in energy production, protein fate, nucleotide biosynthesis, cell division, transport and binding, signal transduction, and fatty acid and phospholipid metabolism in the cell. In addition, phenol hydroxylase, thioesterase, zinc metalloprotease, and aspartate kinase were newly expressed after cadmium exposure at the concentration of the LC10 value. Therefore, these proteins seem to be potential biomarkers for cadmium exposure in the aquatic ecosystems.

Original languageEnglish
Pages (from-to)945-957
Number of pages13
JournalProteomics
Volume6
Issue number3
DOIs
Publication statusPublished - 2006 Feb 1

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Chironomidae
Cadmium
Proteomics
Larva
Toxicity
Proteins
Biosynthesis
Aspartate Kinase
Aquatic ecosystems
phenol 2-monooxygenase
Heavy Metals
Ecosystem
Fatty Acids
Adenosylmethionine Decarboxylase
Acyl Carrier Protein
Aquatic organisms
Aquatic Organisms
Signal transduction
Methyltransferases
Metalloproteases

Keywords

  • 2-DE
  • Cadmium
  • Chironomus riparius
  • MALDI-TOF
  • PD-Quest

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Proteomic evaluation of cadmium toxicity on the midge Chironomus riparius Meigen larvae. / Lee, Sung Eun; Yoo, Dong Hun; Son, Jino; Cho, Ki Jong.

In: Proteomics, Vol. 6, No. 3, 01.02.2006, p. 945-957.

Research output: Contribution to journalArticle

Lee, Sung Eun ; Yoo, Dong Hun ; Son, Jino ; Cho, Ki Jong. / Proteomic evaluation of cadmium toxicity on the midge Chironomus riparius Meigen larvae. In: Proteomics. 2006 ; Vol. 6, No. 3. pp. 945-957.
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