Proteomic of identification of annexins, calcium-dependent membrane binding proteins that mediate osmotic stress and abscisic acid signal transduction in arabidopsis

Sumin Lee, Eun Jung Lee, Eun Ju Yang, Ji Eun Lee, Ae Ran Park, Won Hyun Song, Ohkmae Kim

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

Comparative proteomic analysis of the Arabidopsis thaliana root microsomal fraction was performed to identify novel components of salt stress signaling. Among the salt-responsive microsomal proteins, two spots that increased upon salt treatment on a two-dimensional gel were identified as the same protein, designated annexin 1 (AnnAt1). Annexins comprise a multigene family of Ca 2+-dependent membrane binding proteins and have been extensively studied in animal cells. AnnAt1 is strongly expressed in root but rarely in flower tissue. In this study, the results suggest that salt stress induces translocation from the cytosol to the membrane and potential turnover of existing protein. This process is blocked by EGTA treatment, implying that AnnAt1 functions in stress response are tightly associated with Ca2+. T-DNA insertion mutants of annAt1 and a different isoform, annAt4, displayed hypersensitivity to osmotic stress and abscisic acid (ABA) during germination and early seedling growth. The results collectively suggest that AnnAt1 and AnnAt4 play important roles in osmotic stress and ABA signaling in a Ca 2+-dependent manner.

Original languageEnglish
Pages (from-to)1378-1391
Number of pages14
JournalPlant Cell
Volume16
Issue number6
DOIs
Publication statusPublished - 2004 Jun 1
Externally publishedYes

Fingerprint

Annexins
Signal transduction
Abscisic Acid
Osmotic Pressure
osmotic stress
Arabidopsis
membrane proteins
Proteomics
proteomics
salt stress
signal transduction
abscisic acid
binding proteins
Signal Transduction
Carrier Proteins
Membrane Proteins
Salts
Calcium
salts
Membranes

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Proteomic of identification of annexins, calcium-dependent membrane binding proteins that mediate osmotic stress and abscisic acid signal transduction in arabidopsis. / Lee, Sumin; Lee, Eun Jung; Yang, Eun Ju; Lee, Ji Eun; Park, Ae Ran; Song, Won Hyun; Kim, Ohkmae.

In: Plant Cell, Vol. 16, No. 6, 01.06.2004, p. 1378-1391.

Research output: Contribution to journalArticle

Lee, Sumin ; Lee, Eun Jung ; Yang, Eun Ju ; Lee, Ji Eun ; Park, Ae Ran ; Song, Won Hyun ; Kim, Ohkmae. / Proteomic of identification of annexins, calcium-dependent membrane binding proteins that mediate osmotic stress and abscisic acid signal transduction in arabidopsis. In: Plant Cell. 2004 ; Vol. 16, No. 6. pp. 1378-1391.
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