Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells

Han Na Lee, Soo Hyun Park, Eun Kyung Lee, Raymundo Bernardo, Chan Wha Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the stemness and tumorigenic properties of these cells.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume427
Issue number1
DOIs
Publication statusPublished - 2012 Oct 12

Fingerprint

Neoplastic Stem Cells
Stem cells
Proteomics
Tumors
Colorectal Neoplasms
Actin-Related Protein 2-3 Complex
Cells
Profilins
Proteins
HT29 Cells
Population
Neoplasms
Neoplasm Metastasis

Keywords

  • Cancer stem cell
  • CD133
  • Colorectal cancer
  • Proteomics
  • Two dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells. / Lee, Han Na; Park, Soo Hyun; Lee, Eun Kyung; Bernardo, Raymundo; Kim, Chan Wha.

In: Biochemical and Biophysical Research Communications, Vol. 427, No. 1, 12.10.2012, p. 171-177.

Research output: Contribution to journalArticle

Lee, Han Na ; Park, Soo Hyun ; Lee, Eun Kyung ; Bernardo, Raymundo ; Kim, Chan Wha. / Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 427, No. 1. pp. 171-177.
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