Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells

Han Na Lee, Soo Hyun Park, Eun Kyung Lee, Raymundo Bernardo, Chan Wha Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the stemness and tumorigenic properties of these cells.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalBiochemical and biophysical research communications
Volume427
Issue number1
DOIs
Publication statusPublished - 2012 Oct 12

Keywords

  • CD133
  • Cancer stem cell
  • Colorectal cancer
  • Proteomics
  • Two dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells'. Together they form a unique fingerprint.

  • Cite this