Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells

Han Na Lee; Soo Hyun Park; Eun Kyung Lee; Raymundo Bernardo; Chan Wha Kim

doi:10.1016/j.bbrc.2012.09.036

Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells

Han Na Lee, Soo Hyun Park, Eun Kyung Lee, Raymundo Bernardo, Chan Wha Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the stemness and tumorigenic properties of these cells.

Original language	English
Pages (from-to)	171-177
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	427
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2012.09.036
Publication status	Published - 2012 Oct 12

Keywords

CD133
Cancer stem cell
Colorectal cancer
Proteomics
Two dimensional gel electrophoresis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2012.09.036

Cite this

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title = "Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells",

abstract = "Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the stemness and tumorigenic properties of these cells.",

keywords = "CD133, Cancer stem cell, Colorectal cancer, Proteomics, Two dimensional gel electrophoresis",

author = "Lee, {Han Na} and Park, {Soo Hyun} and Lee, {Eun Kyung} and Raymundo Bernardo and Kim, {Chan Wha}",

note = "Funding Information: This study was supported by BK21 (Brain Korea 21). We would like to thank the Center for Analytical and Lifescience Instruments of Korea University. ",

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AU - Lee, Han Na

AU - Park, Soo Hyun

AU - Lee, Eun Kyung

AU - Bernardo, Raymundo

AU - Kim, Chan Wha

N1 - Funding Information: This study was supported by BK21 (Brain Korea 21). We would like to thank the Center for Analytical and Lifescience Instruments of Korea University.

PY - 2012/10/12

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AB - Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the stemness and tumorigenic properties of these cells.

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KW - Two dimensional gel electrophoresis

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Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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