Proteomic study reveals photosynthesis as downstream of both MAP kinase and cAMP signaling pathways in Chlamydomonas reinhardtii

C. Lee, J. K. Rhee, D. G. Kim, Yoon-E Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Previously, our data indicated that both cAMP and MAP kinase signaling play important roles in microalgal physiology as well as in lipid or carotenoid biosynthesis. In order to understand downstream genes of these signaling pathways, we employed proteomics approach. Both signal pathways were first altered with specific signaling inhibitors or modulators. Treatment of specific inhibitors changed microalgal size and increased lipid contents. With the microalgal cells after treatments of specific signaling inhibitor or modulators, we performed the proteomics analysis to identify downstream genes responsible for these phenotypes. Interestingly, multiple photosynthesis genes were identified, particularly proteins associated with PSII. Our data suggested that MAP kinase and cAMP signaling affect the photosynthesis, thereby leading to microalgal lipid or carotenoid biosynthesis.

Original languageEnglish
Pages (from-to)625-629
Number of pages5
JournalPhotosynthetica
Volume53
Issue number4
DOIs
Publication statusPublished - 2015 Dec 1

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Keywords

  • gene regulation
  • mass spectrometry
  • two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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