Proteomics of Halophilic archaea

Won A. Joo, Chan Wha Kim

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Halophilic archaea is a member of the Halobacteriacea family, the only family in the Halobacteriales order. Most Halophilic archaea require 1.5 M NaCl both to grow and retain the structural integrity of the cells. The proteins of these organisms have thus been adapted to be active and stable in the hypersaline condition. Consequently, the unique properties of these biocatalysts have resulted in several novel applications in industrial processes. Halophilic archaea are also to be useful for bioremediation of hypersaline environment. Proteome data have expended enormously with the significant advance recently achieved in two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The whole genome sequencing of Halobacterium species NRC-1 was completed and this would also provide tremendous help to analyze the protein mass data from the similar strain Halobacterium salinarum. Proteomics coupled with genomic databases now has become a basic tool to understand or identify the function of genes and proteins. In addition, the bioinformatics approach will facilitate to predict the function of novel proteins of Halophilic archaea. This review will discuss current proteome study of Halophilic archaea and introduce the efficient procedures for screening, predicting, and confirming the function of novel halophilic enzymes.

Original languageEnglish
Pages (from-to)237-250
Number of pages14
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume815
Issue number1-2
DOIs
Publication statusPublished - 2005 Feb 5

Fingerprint

Archaea
Proteomics
Proteome
Proteins
Halobacteriales
Halobacterium
Bioremediation
Halobacterium salinarum
Structural integrity
Enzymes
Bioinformatics
Electrophoresis
Environmental Biodegradation
Mass spectrometry
Electrophoresis, Gel, Two-Dimensional
Screening
Computational Biology
Genes
Gels
Mass Spectrometry

Keywords

  • Bioinformatics
  • Enzymes
  • Halophilic archaea
  • Prediction of function
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Proteomics of Halophilic archaea. / Joo, Won A.; Kim, Chan Wha.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 815, No. 1-2, 05.02.2005, p. 237-250.

Research output: Contribution to journalArticle

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