Proximity Labeling Techniques: A Multi-Omics Toolbox

Olha Shkel, Yevheniia Kharkivska, Yun Kyung Kim, Jun Seok Lee

Research output: Contribution to journalReview articlepeer-review

2 Citations (Scopus)


Proximity labeling techniques are emerging high-throughput methods for studying protein-protein, protein-RNA, and protein-DNA interactions with temporal and spatial precision. Proximity labeling methods take advantage of enzymes that can covalently label biomolecules with reactive substrates. These labeled biomolecules can be identified using mass spectrometry or next-generation sequencing. The main advantage of these methods is their ability to capture weak or transient interactions between biomolecules. Proximity labeling is indispensable for studying organelle interactomes. Additionally, it can be used to resolve spatial composition of macromolecular complexes. Many of these methods have only recently been introduced; nonetheless, they have already provided new and deep insights into the biological processes at the cellular, organ, and organism levels. In this paper, we review a broad range of proximity labeling techniques, their development, drawbacks and advantages, and implementations in recent studies.

Original languageEnglish
Article numbere202101240
JournalChemistry - An Asian Journal
Issue number2
Publication statusPublished - 2022 Jan 17

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry


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