PSA-NCAM-negative neural crest cells emerging during neural induction of pluripotent stem cells cause mesodermal tumors and unwanted grafts

Dongjin R. Lee, Jeong Eun Yoo, Jae Souk Lee, Sanghyun Park, Junwon Lee, Chul Yong Park, Eunhyun Ji, Han Soo Kim, Dong Youn Hwang, Dae-Sung Kim, Dong Wook Kim

Research output: Contribution to journalArticle

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Abstract

Tumorigenic potential of human pluripotent stem cells (hPSCs) is an important issue in clinical applications. Despite many efforts, PSC-derived neural precursor cells (NPCs) have repeatedly induced tumors in animal models even though pluripotent cells were not detected. We found that polysialic acid-neural cell adhesion molecule (PSA-NCAM) - cells among the early NPCs caused tumors, whereas PSA-NCAM + cells were nontumorigenic. Molecular profiling, global gene analysis, and multilineage differentiation of PSA-NCAM - cells confirm that they are multipotent neural crest stem cells (NCSCs) that could differentiate into both ectodermal and mesodermal lineages. Transplantation of PSA-NCAM - cells in a gradient manner mixed with PSA-NCAM + cells proportionally increased mesodermal tumor formation and unwanted grafts such as PERIPHERIN + cells or pigmented cells in the rat brain. Therefore, we suggest that NCSCs are a critical target for tumor prevention in hPSC-derived NPCs, and removal of PSA-NCAM - cells eliminates the tumorigenic potential originating from NCSCs after transplantation.

Original languageEnglish
Pages (from-to)821-834
Number of pages14
JournalStem Cell Reports
Volume4
Issue number5
DOIs
Publication statusPublished - 2015 May 12

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Neural Cell Adhesion Molecules
Pluripotent Stem Cells
Neural Crest
Stem cells
Grafts
Tumors
Transplants
Neoplasms
Neural Stem Cells
polysialic acid
Rats
Brain
Animals
Genes
Multipotent Stem Cells
Stem Cell Transplantation
Animal Models

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

PSA-NCAM-negative neural crest cells emerging during neural induction of pluripotent stem cells cause mesodermal tumors and unwanted grafts. / Lee, Dongjin R.; Yoo, Jeong Eun; Lee, Jae Souk; Park, Sanghyun; Lee, Junwon; Park, Chul Yong; Ji, Eunhyun; Kim, Han Soo; Hwang, Dong Youn; Kim, Dae-Sung; Kim, Dong Wook.

In: Stem Cell Reports, Vol. 4, No. 5, 12.05.2015, p. 821-834.

Research output: Contribution to journalArticle

Lee, Dongjin R. ; Yoo, Jeong Eun ; Lee, Jae Souk ; Park, Sanghyun ; Lee, Junwon ; Park, Chul Yong ; Ji, Eunhyun ; Kim, Han Soo ; Hwang, Dong Youn ; Kim, Dae-Sung ; Kim, Dong Wook. / PSA-NCAM-negative neural crest cells emerging during neural induction of pluripotent stem cells cause mesodermal tumors and unwanted grafts. In: Stem Cell Reports. 2015 ; Vol. 4, No. 5. pp. 821-834.
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