Pseudarthrobacter psychrotolerans sp. Nov., a cold-adapted bacterium isolated from antarctic soil

Yoonjae Shin, Byoung Hee Lee, Ki Eun Lee, Woojun Park

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Abstract

A novel cold-tolerant bacterium, designated strain YJ56T, was isolated from Antarctic soil collected from the Cape Burk area. Phylogenetic analysis through 16S rRNA gene sequence similarity revealed that strain YJ56T was most closely related to the genus Pseudarthrobacter, including Pseudarthrobacter oxydans DSM 20119T (99.06% similarity), Pseudarthrobacter polychromogenes DSM 20136T (98.98%) and Pseudarthrobacter sulfonivorans ALLT (98.76%). The genome size (5.2 Mbp) of strain YJ56T was the largest among all the published genomes of Pseudarthrobacter type strains (4.2–5.0 Mbp). The genomic G+C content of strain YJ56T (64.7mol%) was found to be consistent with those of other Pseudarthrobacter strains (62.0–71.0mol%). The average nucleotide identity and average amino acid identity values between strain YJ56T and P. sulfonivorans ALLT were estimated at 84.1 and 84.2%, respectively. The digital DNA–DNA hybridization value between the two strains was calculated to be 28.0%. This rod-shaped and obligate aerobic strain exhibited no swimming or swarming motility. It had catalase activity but no oxidase activity. Cells grew at 4–28 °C (optimum, 13 °C) and pH 5.0–11.0 (optimum, pH 7.0) and with 0–6.0% (w/v) NaCl (optimum, 0%) in Reasoner's 2A medium. MK-9 (H2) was the sole menaquinone. Two-dimensional TLC results revealed that the primary polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two glycolipids and phosphatidylinositol. Fatty acid methyl ester analysis showed that anteiso-C15:0, anteiso-C17:0, iso-C15:0, C16:0 and iso-C16:0 were the major cellular fatty acids in strain YJ56T. Based on phenotypic and genotypic characteristics, strain YJ56T represents a novel species of the genus Pseudarthrobacter, and thus the name Pseudarthrobacter psychrotolerans sp. nov is proposed. The type strain is YJ56T (=JCM 33881T=KACC 21510T).

Original languageEnglish
Article number004505
Pages (from-to)6106-6114
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number12
DOIs
Publication statusPublished - 2020

Keywords

  • Actinobacteria
  • Arthrobacter
  • Menaquinone 9
  • Micrococcaceae
  • Psychrophilic
  • Taxonomy

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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