Pseudokineococcus basanitobsidens sp. Nov., isolated from volcanic rock

A novel Gram-strain-positive, non-spore-forming bacterial strain, designated SKC1-2^T, was isolated from volcanic rock of the scoria cone of Seobjikoji, Jeju, Republic of Korea. Cells were aerobic, catalase-positive, oxidase-negative, motile and cocci. Colonies of cells were dark orange-coloured, circular, smooth and convex. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was related to members of the genus Pseudokineococcus. Phylogenetic neighbours were P. marinus KCCM 42250^T (98.2 %, 16S rRNA gene sequence similarity) and P. lusitanus DSM 23768^T (98.0 %). The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant respiratory quinone was MK-9 (H₂). The predominant respiratory quinone was MK-9(H₂) and the major fatty acid was anteiso-C_{15: 0}. The polar lipid profile included major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and two unidentified phosphoglycolipids. The DNA G+C content was 74.9 mol%. DNA–DNA relatedness values between strain SKC1-2^T and P. lusitanus DSM 23768^T or P. marinus KCCM 42250^T were 37.5–38.1% or 45.4–46.4 %, respectively. On the basis of the phenotypic differences and DNA–DNA relatedness data, the isolate represents a new species of the genus Pseudokineococcus, for which the name Pseudokineococcus basanitobsidens sp. nov. is proposed. The type strain is SKC1-2^T (=DSM 103726^T=KCCM 43221^T).