Abstract
A novel Gram-strain-positive, non-spore-forming bacterial strain, designated SKC1-2T, was isolated from volcanic rock of the scoria cone of Seobjikoji, Jeju, Republic of Korea. Cells were aerobic, catalase-positive, oxidase-negative, motile and cocci. Colonies of cells were dark orange-coloured, circular, smooth and convex. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was related to members of the genus Pseudokineococcus. Phylogenetic neighbours were P. marinus KCCM 42250T (98.2 %, 16S rRNA gene sequence similarity) and P. lusitanus DSM 23768T (98.0 %). The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant respiratory quinone was MK-9 (H2). The predominant respiratory quinone was MK-9(H2) and the major fatty acid was anteiso-C15: 0. The polar lipid profile included major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and two unidentified phosphoglycolipids. The DNA G+C content was 74.9 mol%. DNA–DNA relatedness values between strain SKC1-2T and P. lusitanus DSM 23768T or P. marinus KCCM 42250T were 37.5–38.1% or 45.4–46.4 %, respectively. On the basis of the phenotypic differences and DNA–DNA relatedness data, the isolate represents a new species of the genus Pseudokineococcus, for which the name Pseudokineococcus basanitobsidens sp. nov. is proposed. The type strain is SKC1-2T (=DSM 103726T=KCCM 43221T).
| Original language | English |
|---|---|
| Article number | 002206 |
| Pages (from-to) | 3824-3828 |
| Number of pages | 5 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 67 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2017 Oct 1 |
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Keywords
- Actinobacteria
- DNA–DNA hybridization
- Polyphasic taxonomic characterization
- Pseudokineococcus
- Volcanic rock
ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Pseudokineococcus basanitobsidens sp. Nov., isolated from volcanic rock. / Lee, Dong Wan; Park, Min Young; Kim, Jae-Jin; Kim, Beom Seok.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 10, 002206, 01.10.2017, p. 3824-3828.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pseudokineococcus basanitobsidens sp. Nov., isolated from volcanic rock
AU - Lee, Dong Wan
AU - Park, Min Young
AU - Kim, Jae-Jin
AU - Kim, Beom Seok
PY - 2017/10/1
Y1 - 2017/10/1
N2 - A novel Gram-strain-positive, non-spore-forming bacterial strain, designated SKC1-2T, was isolated from volcanic rock of the scoria cone of Seobjikoji, Jeju, Republic of Korea. Cells were aerobic, catalase-positive, oxidase-negative, motile and cocci. Colonies of cells were dark orange-coloured, circular, smooth and convex. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was related to members of the genus Pseudokineococcus. Phylogenetic neighbours were P. marinus KCCM 42250T (98.2 %, 16S rRNA gene sequence similarity) and P. lusitanus DSM 23768T (98.0 %). The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant respiratory quinone was MK-9 (H2). The predominant respiratory quinone was MK-9(H2) and the major fatty acid was anteiso-C15: 0. The polar lipid profile included major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and two unidentified phosphoglycolipids. The DNA G+C content was 74.9 mol%. DNA–DNA relatedness values between strain SKC1-2T and P. lusitanus DSM 23768T or P. marinus KCCM 42250T were 37.5–38.1% or 45.4–46.4 %, respectively. On the basis of the phenotypic differences and DNA–DNA relatedness data, the isolate represents a new species of the genus Pseudokineococcus, for which the name Pseudokineococcus basanitobsidens sp. nov. is proposed. The type strain is SKC1-2T (=DSM 103726T=KCCM 43221T).
AB - A novel Gram-strain-positive, non-spore-forming bacterial strain, designated SKC1-2T, was isolated from volcanic rock of the scoria cone of Seobjikoji, Jeju, Republic of Korea. Cells were aerobic, catalase-positive, oxidase-negative, motile and cocci. Colonies of cells were dark orange-coloured, circular, smooth and convex. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was related to members of the genus Pseudokineococcus. Phylogenetic neighbours were P. marinus KCCM 42250T (98.2 %, 16S rRNA gene sequence similarity) and P. lusitanus DSM 23768T (98.0 %). The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant respiratory quinone was MK-9 (H2). The predominant respiratory quinone was MK-9(H2) and the major fatty acid was anteiso-C15: 0. The polar lipid profile included major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and two unidentified phosphoglycolipids. The DNA G+C content was 74.9 mol%. DNA–DNA relatedness values between strain SKC1-2T and P. lusitanus DSM 23768T or P. marinus KCCM 42250T were 37.5–38.1% or 45.4–46.4 %, respectively. On the basis of the phenotypic differences and DNA–DNA relatedness data, the isolate represents a new species of the genus Pseudokineococcus, for which the name Pseudokineococcus basanitobsidens sp. nov. is proposed. The type strain is SKC1-2T (=DSM 103726T=KCCM 43221T).
KW - Actinobacteria
KW - DNA–DNA hybridization
KW - Polyphasic taxonomic characterization
KW - Pseudokineococcus
KW - Volcanic rock
UR - http://www.scopus.com/inward/record.url?scp=85031774181&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85031774181&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002206
DO - 10.1099/ijsem.0.002206
M3 - Article
VL - 67
SP - 3824
EP - 3828
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
SN - 1466-5026
IS - 10
M1 - 002206
ER -