Pseudokineococcus basanitobsidens sp. Nov., isolated from volcanic rock

Dong Wan Lee, Min Young Park, Jae-Jin Kim, Beom Seok Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A novel Gram-strain-positive, non-spore-forming bacterial strain, designated SKC1-2T, was isolated from volcanic rock of the scoria cone of Seobjikoji, Jeju, Republic of Korea. Cells were aerobic, catalase-positive, oxidase-negative, motile and cocci. Colonies of cells were dark orange-coloured, circular, smooth and convex. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was related to members of the genus Pseudokineococcus. Phylogenetic neighbours were P. marinus KCCM 42250T (98.2 %, 16S rRNA gene sequence similarity) and P. lusitanus DSM 23768T (98.0 %). The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant respiratory quinone was MK-9 (H2). The predominant respiratory quinone was MK-9(H2) and the major fatty acid was anteiso-C15: 0. The polar lipid profile included major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and two unidentified phosphoglycolipids. The DNA G+C content was 74.9 mol%. DNA–DNA relatedness values between strain SKC1-2T and P. lusitanus DSM 23768T or P. marinus KCCM 42250T were 37.5–38.1% or 45.4–46.4 %, respectively. On the basis of the phenotypic differences and DNA–DNA relatedness data, the isolate represents a new species of the genus Pseudokineococcus, for which the name Pseudokineococcus basanitobsidens sp. nov. is proposed. The type strain is SKC1-2T (=DSM 103726T=KCCM 43221T).

Original languageEnglish
Article number002206
Pages (from-to)3824-3828
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number10
DOIs
Publication statusPublished - 2017 Oct 1

Fingerprint

volcanic rocks
rRNA Genes
relatedness
volcanic rock
Diaminopimelic Acid
phylogenetics
Republic of Korea
Phosphatidylglycerols
Cardiolipins
Peptidoglycan
gene
acid
Base Composition
Phosphatidylinositols
phospholipid
quinones
Catalase
Cell Wall
Names
Phospholipids

Keywords

  • Actinobacteria
  • DNA–DNA hybridization
  • Polyphasic taxonomic characterization
  • Pseudokineococcus
  • Volcanic rock

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Pseudokineococcus basanitobsidens sp. Nov., isolated from volcanic rock. / Lee, Dong Wan; Park, Min Young; Kim, Jae-Jin; Kim, Beom Seok.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 10, 002206, 01.10.2017, p. 3824-3828.

Research output: Contribution to journalArticle

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abstract = "A novel Gram-strain-positive, non-spore-forming bacterial strain, designated SKC1-2T, was isolated from volcanic rock of the scoria cone of Seobjikoji, Jeju, Republic of Korea. Cells were aerobic, catalase-positive, oxidase-negative, motile and cocci. Colonies of cells were dark orange-coloured, circular, smooth and convex. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was related to members of the genus Pseudokineococcus. Phylogenetic neighbours were P. marinus KCCM 42250T (98.2 {\%}, 16S rRNA gene sequence similarity) and P. lusitanus DSM 23768T (98.0 {\%}). The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant respiratory quinone was MK-9 (H2). The predominant respiratory quinone was MK-9(H2) and the major fatty acid was anteiso-C15: 0. The polar lipid profile included major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and two unidentified phosphoglycolipids. The DNA G+C content was 74.9 mol{\%}. DNA–DNA relatedness values between strain SKC1-2T and P. lusitanus DSM 23768T or P. marinus KCCM 42250T were 37.5–38.1{\%} or 45.4–46.4 {\%}, respectively. On the basis of the phenotypic differences and DNA–DNA relatedness data, the isolate represents a new species of the genus Pseudokineococcus, for which the name Pseudokineococcus basanitobsidens sp. nov. is proposed. The type strain is SKC1-2T (=DSM 103726T=KCCM 43221T).",
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