Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor

K. Kohri, I. F. Ueki, Jae Jeong Shim, P. R. Burgel, Y. M. Oh, D. C. Tam, T. Dao-Pick, J. A. Nadel

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Hypersecretory disease associated with Pseudomonas aeruginosa (PA) infections is characterised by increased goblet cells and increased mucin production. Recently, an epidermal growth factor receptor (EGFR) signalling cascade was shown to be a common pathway through which many stimuli induce mucin MUC5AC expression in airways by differentiation to a goblet cell phenotype. This study looked at whether PA products induce EGFR expression and activation and thus result in mucin MUC5AC production. Human airway epithelial (NCI-H292) cells were stimulated with PA culture supernatant (Sup). MUC5AC protein production, MUC5AC and EGFR messenger ribonucleic acid (mRNA) expression, and phosphorylated EGFR and phosphorylated p44/42 mitogen-activated protein kinase (MAPK) were all examined using enzyme-linked immunosorbent assay, by in situ hybridisation and by immunoblotting. PA Sup induced MUC5AC mRNA and subsequent protein expression, EGFR and p44/42 MAPK phosphorylation and EGFR mRNA expression. Induction of MUC5AC mRNA and protein expression and EGFR and p44/42 MAPK phosphorylation were inhibited completely by pretreatment with a selective EGFR tyrosine kinase inhibitor. Pretreatment with a selective inhibitor of MAPK kinase prevented MUC5AC production and p44/42 MAPK phosphorylation but not EGFR phosphorylation. The authors conclude that PA products induce mucin MUC5AC production in human airway epithelial cells via the expression and activation of epidermal growth factor receptor.

Original languageEnglish
Pages (from-to)1263-1270
Number of pages8
JournalEuropean Respiratory Journal
Volume20
Issue number5
DOIs
Publication statusPublished - 2002 Nov 1
Externally publishedYes

Fingerprint

Epidermal Growth Factor Receptor
Pseudomonas aeruginosa
Mucins
Mitogen-Activated Protein Kinases
Phosphorylation
RNA
Goblet Cells
Pseudomonas Infections
Proteins
Mitogen-Activated Protein Kinase Kinases
Immunoblotting
Protein-Tyrosine Kinases
In Situ Hybridization
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
Phenotype

Keywords

  • Airway epithelium
  • Mucin production
  • Tyrosine kinase phosphorylation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor. / Kohri, K.; Ueki, I. F.; Shim, Jae Jeong; Burgel, P. R.; Oh, Y. M.; Tam, D. C.; Dao-Pick, T.; Nadel, J. A.

In: European Respiratory Journal, Vol. 20, No. 5, 01.11.2002, p. 1263-1270.

Research output: Contribution to journalArticle

Kohri, K, Ueki, IF, Shim, JJ, Burgel, PR, Oh, YM, Tam, DC, Dao-Pick, T & Nadel, JA 2002, 'Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor', European Respiratory Journal, vol. 20, no. 5, pp. 1263-1270. https://doi.org/10.1183/09031936.02.00001402
Kohri, K. ; Ueki, I. F. ; Shim, Jae Jeong ; Burgel, P. R. ; Oh, Y. M. ; Tam, D. C. ; Dao-Pick, T. ; Nadel, J. A. / Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor. In: European Respiratory Journal. 2002 ; Vol. 20, No. 5. pp. 1263-1270.
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