Pseudomonas neustonica sp. Nov., isolated from the sea surface microlayer of the ross sea (antarctica)

Gwang Il Jang, Inae Lee, Tran Thu Ha, Soo Jung Yoon, Yeon Ju Hwang, Hana Yi, Sukyoung Yun, Won Sang Lee, Chung Yeon Hwang

Research output: Contribution to journalArticle

Abstract

Gram-stain-negative, aerobic and rod-shaped bacterial strains, designated SSM26T and SSM44, were isolated from a sea surface microlayer sample from the Ross Sea, Antarctica. Analysis of the 16S rRNA gene sequences of strains SSM26T and SSM44 revealed a clear affiliation with the genus Pseudomonas. Based on the results of phylogenetic analysis, strains SSM26T and SSM44 showed the closest phylogenetic relationship with the species Pseudomonas sabulinigri KCTC 22137T with the 16S rRNA gene sequence similarity level of 98.5 %. Strains SSM26T and SSM44 grew optimally at 30 °C, pH 7.0–7.5 and 0.5– 10.0 % NaCl (w/v). The major cellular fatty acids were C18: 1 ω7c (31.3–34.9%), C16: 0 (15.5–20.2%), summed feature 3 (C16: 1 ω7c/ C16: 1 ω6c; 19.5–25.4 %) and C12: 0 (6.0–9.3 %). The genomic DNA G+C content of each strain was 56.2 mol%. Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strains SSM26T and SSM44 constituted a single species that was clearly distinguishable from its phylogenetically close relatives. The combined phenotypic, chemotaxonomic, genomic and phylogenetic data also showed that strains SSM26T and SSM44 could be distinguished from validly published members of the genus Pseudomonas. Thus, these strains should be classified as representing a novel species in the genus Pseudomonas, for which the name Pseudomonas neustonica sp. nov. is proposed with the type strain SSM26T (=KCCM 43193T=JCM 31284T=PAMC 28426T) and a sister strain SSM44 (=KCCM 43194=JCM 31285=PAMC 28427).

Original languageEnglish
Article number004240
Pages (from-to)3832-3838
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number6
DOIs
Publication statusPublished - 2020

Keywords

  • Antarctica
  • Marine bacteria
  • Pseudomonas
  • Sea surface microlayer

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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