PTRF/cavin-1 is essential for multidrug resistance in cancer cells

Jae Sung Yi, Dong Gi Mun, Hyun Lee, Jun Sub Park, Jung Woo Lee, Jae Seon Lee, Su Jin Kim, Bong-Rae Cho, Sang-Won Lee, Young-Gyu Ko

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Since detergent-resistant lipid rafts play important roles in multidrug resistance (MDR), their comprehensive proteomics could provide new insights to understand the underlying molecular mechanism of MDR in cancer cells. In the present work, lipid rafts were isolated from MCF-7 and adriamycin-resistant MCF-7/ADR cells and their proteomes were analyzed by label-free quantitative proteomics. Polymerase I and transcript release factor (PTRF)/cavin-1 was measured to be upregulated along with multidrug-resistant P-glycoprotein, caveolin-1, and serum deprivation protein response/cavin-2 in the lipid rafts of MCF-7/ADR cells. PTRF knockdown led to reduction in the amount of lipid rafts on the surface of MCF7/ADR cells as determined by cellular staining with lipid raft-specific dyes such as S-laurdan2 and FITC-conjugated cholera toxin B. PTRF knockdown also reduced MDR in MCF-7/ADR cells. These data indicate that PTRF is necessary for MDR in cancer cells via the fortification of lipid rafts.

Original languageEnglish
Pages (from-to)605-614
Number of pages10
JournalJournal of Proteome Research
Volume12
Issue number2
DOIs
Publication statusPublished - 2013 Feb 1

Fingerprint

Multiple Drug Resistance
MCF-7 Cells
Cells
Lipids
Neoplasms
Proteomics
Caveolin 1
Fluorescein-5-isothiocyanate
Cholera Toxin
P-Glycoprotein
Proteome
Detergents
Doxorubicin
Caviner
Labels
Blood Proteins
Coloring Agents
Staining and Labeling
Proteins

Keywords

  • cavin-1
  • label-free quantitative proteomics
  • lipid rafts
  • multidrug resistance
  • PTRF

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Yi, J. S., Mun, D. G., Lee, H., Park, J. S., Lee, J. W., Lee, J. S., ... Ko, Y-G. (2013). PTRF/cavin-1 is essential for multidrug resistance in cancer cells. Journal of Proteome Research, 12(2), 605-614. https://doi.org/10.1021/pr300651m

PTRF/cavin-1 is essential for multidrug resistance in cancer cells. / Yi, Jae Sung; Mun, Dong Gi; Lee, Hyun; Park, Jun Sub; Lee, Jung Woo; Lee, Jae Seon; Kim, Su Jin; Cho, Bong-Rae; Lee, Sang-Won; Ko, Young-Gyu.

In: Journal of Proteome Research, Vol. 12, No. 2, 01.02.2013, p. 605-614.

Research output: Contribution to journalArticle

Yi, Jae Sung ; Mun, Dong Gi ; Lee, Hyun ; Park, Jun Sub ; Lee, Jung Woo ; Lee, Jae Seon ; Kim, Su Jin ; Cho, Bong-Rae ; Lee, Sang-Won ; Ko, Young-Gyu. / PTRF/cavin-1 is essential for multidrug resistance in cancer cells. In: Journal of Proteome Research. 2013 ; Vol. 12, No. 2. pp. 605-614.
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