Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing

Yue Chen, Aram Chung, Ting Hsiang Wu, Michael A. Teitell, Dino Di Carlo, Pei Yu Chiou

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s -1 without severe dilution effects from high volume sheath flows.

Original languageEnglish
Pages (from-to)1746-1751
Number of pages6
JournalSmall
Volume10
Issue number9
DOIs
Publication statusPublished - 2014 May 14
Externally publishedYes

Fingerprint

Pulsed lasers
Sorting
Lasers
Cells
Throughput
Dilution
Costs and Cost Analysis
Costs

Keywords

  • cell sorting
  • inertial focusing
  • lab-on-a-chip devices
  • lasers
  • microfluidics

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Cite this

Chen, Y., Chung, A., Wu, T. H., Teitell, M. A., Di Carlo, D., & Chiou, P. Y. (2014). Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing. Small, 10(9), 1746-1751. https://doi.org/10.1002/smll.201302885

Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing. / Chen, Yue; Chung, Aram; Wu, Ting Hsiang; Teitell, Michael A.; Di Carlo, Dino; Chiou, Pei Yu.

In: Small, Vol. 10, No. 9, 14.05.2014, p. 1746-1751.

Research output: Contribution to journalArticle

Chen, Y, Chung, A, Wu, TH, Teitell, MA, Di Carlo, D & Chiou, PY 2014, 'Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing', Small, vol. 10, no. 9, pp. 1746-1751. https://doi.org/10.1002/smll.201302885
Chen, Yue ; Chung, Aram ; Wu, Ting Hsiang ; Teitell, Michael A. ; Di Carlo, Dino ; Chiou, Pei Yu. / Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing. In: Small. 2014 ; Vol. 10, No. 9. pp. 1746-1751.
@article{1a5e5063b017484388f56c5152e53bdf,
title = "Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing",
abstract = "A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90{\%} is achieved at a throughput of 10 000 particles s -1 without severe dilution effects from high volume sheath flows.",
keywords = "cell sorting, inertial focusing, lab-on-a-chip devices, lasers, microfluidics",
author = "Yue Chen and Aram Chung and Wu, {Ting Hsiang} and Teitell, {Michael A.} and {Di Carlo}, Dino and Chiou, {Pei Yu}",
year = "2014",
month = "5",
day = "14",
doi = "10.1002/smll.201302885",
language = "English",
volume = "10",
pages = "1746--1751",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley-VCH Verlag",
number = "9",

}

TY - JOUR

T1 - Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing

AU - Chen, Yue

AU - Chung, Aram

AU - Wu, Ting Hsiang

AU - Teitell, Michael A.

AU - Di Carlo, Dino

AU - Chiou, Pei Yu

PY - 2014/5/14

Y1 - 2014/5/14

N2 - A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s -1 without severe dilution effects from high volume sheath flows.

AB - A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s -1 without severe dilution effects from high volume sheath flows.

KW - cell sorting

KW - inertial focusing

KW - lab-on-a-chip devices

KW - lasers

KW - microfluidics

UR - http://www.scopus.com/inward/record.url?scp=84899823029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899823029&partnerID=8YFLogxK

U2 - 10.1002/smll.201302885

DO - 10.1002/smll.201302885

M3 - Article

VL - 10

SP - 1746

EP - 1751

JO - Small

JF - Small

SN - 1613-6810

IS - 9

ER -