Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing

Yue Chen, Aram J. Chung, Ting Hsiang Wu, Michael A. Teitell, Dino Di Carlo, Pei Yu Chiou

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s-1 without severe dilution effects from high volume sheath flows.

Original languageEnglish
Pages (from-to)1746-1751
Number of pages6
JournalSmall
Volume10
Issue number9
DOIs
Publication statusPublished - 2014 May 14
Externally publishedYes

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Keywords

  • cell sorting
  • inertial focusing
  • lab-on-a-chip devices
  • lasers
  • microfluidics

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Cite this

Chen, Y., Chung, A. J., Wu, T. H., Teitell, M. A., Di Carlo, D., & Chiou, P. Y. (2014). Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing. Small, 10(9), 1746-1751. https://doi.org/10.1002/smll.201302885