Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing

Yue Chen, Aram J. Chung, Ting Hsiang Wu, Michael A. Teitell, Dino Di Carlo, Pei Yu Chiou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a Pulsed Laser Activated Cell Sorter (PLACS) integrated with 3D sheathless inertial focusing that is capable to sort at 10,000 particles sec-1 with >90% sort purity and 6,000 cells sec-1 with >80% sort purity. It is realized by exciting laser induced cavitation bubbles in a single layer PDMS microfluidic channel to create high speed liquid jets to deflect detected fluorescent samples. Fluid inertia and secondary flows induced by stepped microchannels are used to focus samples in 3 dimensions. After focusing, samples go through an expansion chamber whose function is to enlarge the separation distance between two closely positioned particles by particle-particle interaction in inertial flows and reduce the coincident events within the switching window (16 μsec in time or 32 μm in distance). This sorter uses 10 times lower initial concentration cell samples than that in sheath-based PLACS in order to avoid severe dilution effects from high volume sheath flows at the same throughput.

Original languageEnglish
Title of host publication9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages315-318
Number of pages4
ISBN (Electronic)9781479947270
DOIs
Publication statusPublished - 2014 Sep 23
Event9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 - Waikiki Beach, United States
Duration: 2014 Apr 132014 Apr 16

Publication series

Name9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

Conference

Conference9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
CountryUnited States
CityWaikiki Beach
Period14/4/1314/4/16

Keywords

  • Cell sorting
  • Inertial focusing
  • Lab-on-a-chip devices
  • Lasers
  • Microfluidics

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Chen, Y., Chung, A. J., Wu, T. H., Teitell, M. A., Di Carlo, D., & Chiou, P. Y. (2014). Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing. In 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 (pp. 315-318). [6908816] (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2014.6908816