Pure enzyme cocktails tailored for the saccharification of sugarcane bagasse pretreated by using different methods

In Jung Kim, Hee Jin Lee, Kyoung Heon Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The compositions and physical properties of pretreated lignocellulose vary depending on pretreatment methods; therefore, enzyme cocktails specific to pretreatments are desired for efficient saccharification of lignocellulose. Here, enzyme cocktails consisting of three pure lignocellulolytic enzymes endoglucanase (EG), cellobiohydrolase (CBH) and endoxylanase (XN) with a fixed amount of β-glucosidase were tailored for acid- and alkali-pretreated sugarcane bagasse (ACID and ALKALI, respectively). Based on a mixture design, the optimal mass ratios of EG, CBH, and XN were determined to be 61.25:38.73:0.02 and 53.99:34.60:11.41 for ACID and ALKALI, respectively. The optimized enzyme cocktail yielded a higher or comparable amount of reducing sugars from the hydrolysis of ACID and ALKALI when compared to that obtained using commercial cellulase mixtures. Using the commercial and easily available pure enzymes, this simple method for the in-house preparation of an enzyme cocktail specific to pretreated lignocellulose consisting of only four enzymes with a high level of hydrolysis will be helpful for achieving enzymatic saccharification in the lignocellulose-based biorefinery.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalProcess Biochemistry
Volume57
DOIs
Publication statusPublished - 2017 Jun 1

Keywords

  • Cellulase cocktail
  • Lignocellulose
  • Mixture design
  • Pretreatment
  • Sugarcane bagasse

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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