Purification and characterization of an extracellular β-glucosidase produced by phoma sp. KCTC11825BP isolated from rotten mandarin peel

Jung Youn Choi, Ah Reum Park, Yong Jin Kim, Jae-Jin Kim, Chang Jun Cha, Jeong Jun Yoon

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7 Citations (Scopus)


A β-glucosidase from Phoma sp. KCTC11825BP isolated from rotten mandarin peel was purified 8.5-fold with a specific activity of 84.5 U/mg protein. The purified enzyme had a molecular mass of 440 kDa with a subunit of 110 kDa. The partial amino acid sequence of the purified β-glucosidase evidenced high homology with the fungal β-glucosidases belonging to glycosyl hydrolase family 3. Its optimal activity was detected at pH 4.5 and 60oC, and the enzyme had a half-life of 53 h at 60oC. The Km values for p-nitrophenyl-β-D-glucopyranoside and cellobiose were 0.3 mM and 3.2 mM, respectively. The enzyme was competitively inhibited by both glucose (Ki=1.7 mM) and glucono-δ-lactone (Ki=0.1 mM) when pNPG was used as the substrate. Its activity was inhibited by 41% by 10 mM Cu2+ and stimulated by 20% by 10 mM Mg2+.

Original languageEnglish
Pages (from-to)503-508
Number of pages6
JournalJournal of Microbiology and Biotechnology
Issue number5
Publication statusPublished - 2011 May 1



  • β-glucosidase
  • Characterization
  • Identification
  • Phoma sp.
  • Purification

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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