Purification and characterization of an extracellular β-glucosidase produced by phoma sp. KCTC11825BP isolated from rotten mandarin peel

Jung Youn Choi, Ah Reum Park, Yong Jin Kim, Jae-Jin Kim, Chang Jun Cha, Jeong Jun Yoon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A β-glucosidase from Phoma sp. KCTC11825BP isolated from rotten mandarin peel was purified 8.5-fold with a specific activity of 84.5 U/mg protein. The purified enzyme had a molecular mass of 440 kDa with a subunit of 110 kDa. The partial amino acid sequence of the purified β-glucosidase evidenced high homology with the fungal β-glucosidases belonging to glycosyl hydrolase family 3. Its optimal activity was detected at pH 4.5 and 60oC, and the enzyme had a half-life of 53 h at 60oC. The Km values for p-nitrophenyl-β-D-glucopyranoside and cellobiose were 0.3 mM and 3.2 mM, respectively. The enzyme was competitively inhibited by both glucose (Ki=1.7 mM) and glucono-δ-lactone (Ki=0.1 mM) when pNPG was used as the substrate. Its activity was inhibited by 41% by 10 mM Cu2+ and stimulated by 20% by 10 mM Mg2+.

Original languageEnglish
Pages (from-to)503-508
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume21
Issue number5
DOIs
Publication statusPublished - 2011 May 1

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Glucosidases
Enzymes
Cellobiose
Hydrolases
Lactones
Half-Life
Amino Acid Sequence
Glucose
Proteins

Keywords

  • β-glucosidase
  • Characterization
  • Identification
  • Phoma sp.
  • Purification

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Purification and characterization of an extracellular β-glucosidase produced by phoma sp. KCTC11825BP isolated from rotten mandarin peel. / Choi, Jung Youn; Park, Ah Reum; Kim, Yong Jin; Kim, Jae-Jin; Cha, Chang Jun; Yoon, Jeong Jun.

In: Journal of Microbiology and Biotechnology, Vol. 21, No. 5, 01.05.2011, p. 503-508.

Research output: Contribution to journalArticle

Choi, Jung Youn ; Park, Ah Reum ; Kim, Yong Jin ; Kim, Jae-Jin ; Cha, Chang Jun ; Yoon, Jeong Jun. / Purification and characterization of an extracellular β-glucosidase produced by phoma sp. KCTC11825BP isolated from rotten mandarin peel. In: Journal of Microbiology and Biotechnology. 2011 ; Vol. 21, No. 5. pp. 503-508.
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