Abstract
Vitellin from the cabbage butterfly Pieris rapae L. was purified and characterized by electrophoresis. Vitellin from P. rapae is a phosphorylated glycolipoprotein of 380,000 ± 10,000 molecular weight as determined by nondenaturing polyacrylamide gel electrophoresis. Two subunits with an Mr of 150,000 and 40,000 were obtained from vitellin. The native molecule is thought to be a tetramer composed of two molecules of each of these subunits. The isoelectric point, as determined by isoelectric focusing on polyacrylamide gels, is 6.10. Vitellin and vitellogenin were indistinguishable by immunological methods such as double diffusion and tandem‐crossed immunoelectrophoresis. Vitellogenin from the hemolymph and vitellin from the ovary were quantified by rocket immunoelectrophoresis. Vitellogenin and vitellin were first detected in 6‐day‐old pupae, and their levels increased continuously during ovarian development. Vitellogenin synthesis by the fat body in 4‐day‐old female pupae could be induced by juvenile hormone I.
| Original language | English |
|---|---|
| Pages (from-to) | 67-79 |
| Number of pages | 13 |
| Journal | Archives of Insect Biochemistry and Physiology |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1988 |
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Keywords
- immunoelectrophoresis
- juvenile hormone
- vitellogenin
ASJC Scopus subject areas
- Physiology
- Biochemistry
- Insect Science
Cite this
Purification, characterization, and synthesis of vitellin from the cabbage butterfly Pieris rapae L. / Kim, Hak R.; Ko, Young-Gyu; Mayer, Richard T.
In: Archives of Insect Biochemistry and Physiology, Vol. 9, No. 1, 1988, p. 67-79.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification, characterization, and synthesis of vitellin from the cabbage butterfly Pieris rapae L.
AU - Kim, Hak R.
AU - Ko, Young-Gyu
AU - Mayer, Richard T.
PY - 1988
Y1 - 1988
N2 - Vitellin from the cabbage butterfly Pieris rapae L. was purified and characterized by electrophoresis. Vitellin from P. rapae is a phosphorylated glycolipoprotein of 380,000 ± 10,000 molecular weight as determined by nondenaturing polyacrylamide gel electrophoresis. Two subunits with an Mr of 150,000 and 40,000 were obtained from vitellin. The native molecule is thought to be a tetramer composed of two molecules of each of these subunits. The isoelectric point, as determined by isoelectric focusing on polyacrylamide gels, is 6.10. Vitellin and vitellogenin were indistinguishable by immunological methods such as double diffusion and tandem‐crossed immunoelectrophoresis. Vitellogenin from the hemolymph and vitellin from the ovary were quantified by rocket immunoelectrophoresis. Vitellogenin and vitellin were first detected in 6‐day‐old pupae, and their levels increased continuously during ovarian development. Vitellogenin synthesis by the fat body in 4‐day‐old female pupae could be induced by juvenile hormone I.
AB - Vitellin from the cabbage butterfly Pieris rapae L. was purified and characterized by electrophoresis. Vitellin from P. rapae is a phosphorylated glycolipoprotein of 380,000 ± 10,000 molecular weight as determined by nondenaturing polyacrylamide gel electrophoresis. Two subunits with an Mr of 150,000 and 40,000 were obtained from vitellin. The native molecule is thought to be a tetramer composed of two molecules of each of these subunits. The isoelectric point, as determined by isoelectric focusing on polyacrylamide gels, is 6.10. Vitellin and vitellogenin were indistinguishable by immunological methods such as double diffusion and tandem‐crossed immunoelectrophoresis. Vitellogenin from the hemolymph and vitellin from the ovary were quantified by rocket immunoelectrophoresis. Vitellogenin and vitellin were first detected in 6‐day‐old pupae, and their levels increased continuously during ovarian development. Vitellogenin synthesis by the fat body in 4‐day‐old female pupae could be induced by juvenile hormone I.
KW - immunoelectrophoresis
KW - juvenile hormone
KW - vitellogenin
UR - http://www.scopus.com/inward/record.url?scp=84990473320&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990473320&partnerID=8YFLogxK
U2 - 10.1002/arch.940090105
DO - 10.1002/arch.940090105
M3 - Article
AN - SCOPUS:84990473320
VL - 9
SP - 67
EP - 79
JO - Archives of Insect Biochemistry and Physiology
JF - Archives of Insect Biochemistry and Physiology
SN - 0739-4462
IS - 1
ER -