Purification of soluble β-glucan with immune-enhancing activity from the cell wall of yeast

Jung Nam Lee, Do Youn Lee, In Hye Ji, Gi Eun Kim, Hye Nam Kim, Jeongwon Sohn, Sangduk Kim, Chan Wha Kim

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

β-Glucan, one of the major cell wall components of Saccharomyces cerevisiae, has been found to enhance immune functions, especially by activating macrophages. However, a major obstacle to the clinical application of β-(1→3)-glucan is its low solubility in aqueous media. In this study, soluble β-glucan, free of mannoprotein, was prepared, and its effects on TNF-α secretion and phagocytosis by macrophages were evaluated. β-Glucan was first rendered soluble from the yeast cell wall by alkaline extraction (glucan-p1). The extract contained 2.8% of protein which was subsequently removed by successive DEAE-cellulose and ConA chromatography. β-Glucan thus prepared was completely free of mannoprotein and was soluble at neutral pH (glucan-p3). The effects of β-glucan on phagocytosis and TNF-α release activity were investigated. While glucan-p1 moderately induced TNF-α secretion at 200 μg/ml (550 pg of TNF-α/5 × 105 cells), glucan-p3 markedly stimulated macrophages at 200 μg/ml (2,860 pg of TNF-α/5 × 105 cells). Furthermore, glucan-p3 stimulated phagocytosis about 20% more than glucan-p1 did. In conclusion, we purified water-soluble β-glucan which was completely devoid of mannoprotein and effectively stimulated the macrophage function, enabling it to be used as an intravenous injection for sepsis.

Original languageEnglish
Pages (from-to)837-841
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number4
DOIs
Publication statusPublished - 2001 Apr 1

Fingerprint

Glucans
Macrophages
glucans
Yeast
Cell Wall
Purification
Yeasts
Cells
cell walls
yeasts
Chromatography
macrophages
phagocytosis
Phagocytosis
Cellulose
Solubility
Proteins
secretion
Water
DEAE-Cellulose Chromatography

Keywords

  • β-glucan
  • Macrophage function
  • Mannoprotein
  • Phagocytosis
  • TNF-α

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Purification of soluble β-glucan with immune-enhancing activity from the cell wall of yeast. / Lee, Jung Nam; Lee, Do Youn; Ji, In Hye; Kim, Gi Eun; Kim, Hye Nam; Sohn, Jeongwon; Kim, Sangduk; Kim, Chan Wha.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 4, 01.04.2001, p. 837-841.

Research output: Contribution to journalArticle

Lee, Jung Nam ; Lee, Do Youn ; Ji, In Hye ; Kim, Gi Eun ; Kim, Hye Nam ; Sohn, Jeongwon ; Kim, Sangduk ; Kim, Chan Wha. / Purification of soluble β-glucan with immune-enhancing activity from the cell wall of yeast. In: Bioscience, Biotechnology and Biochemistry. 2001 ; Vol. 65, No. 4. pp. 837-841.
@article{4a375caa22494ce2ba0daeb5e544a5d5,
title = "Purification of soluble β-glucan with immune-enhancing activity from the cell wall of yeast",
abstract = "β-Glucan, one of the major cell wall components of Saccharomyces cerevisiae, has been found to enhance immune functions, especially by activating macrophages. However, a major obstacle to the clinical application of β-(1→3)-glucan is its low solubility in aqueous media. In this study, soluble β-glucan, free of mannoprotein, was prepared, and its effects on TNF-α secretion and phagocytosis by macrophages were evaluated. β-Glucan was first rendered soluble from the yeast cell wall by alkaline extraction (glucan-p1). The extract contained 2.8{\%} of protein which was subsequently removed by successive DEAE-cellulose and ConA chromatography. β-Glucan thus prepared was completely free of mannoprotein and was soluble at neutral pH (glucan-p3). The effects of β-glucan on phagocytosis and TNF-α release activity were investigated. While glucan-p1 moderately induced TNF-α secretion at 200 μg/ml (550 pg of TNF-α/5 × 105 cells), glucan-p3 markedly stimulated macrophages at 200 μg/ml (2,860 pg of TNF-α/5 × 105 cells). Furthermore, glucan-p3 stimulated phagocytosis about 20{\%} more than glucan-p1 did. In conclusion, we purified water-soluble β-glucan which was completely devoid of mannoprotein and effectively stimulated the macrophage function, enabling it to be used as an intravenous injection for sepsis.",
keywords = "β-glucan, Macrophage function, Mannoprotein, Phagocytosis, TNF-α",
author = "Lee, {Jung Nam} and Lee, {Do Youn} and Ji, {In Hye} and Kim, {Gi Eun} and Kim, {Hye Nam} and Jeongwon Sohn and Sangduk Kim and Kim, {Chan Wha}",
year = "2001",
month = "4",
day = "1",
doi = "10.1271/bbb.65.837",
language = "English",
volume = "65",
pages = "837--841",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "4",

}

TY - JOUR

T1 - Purification of soluble β-glucan with immune-enhancing activity from the cell wall of yeast

AU - Lee, Jung Nam

AU - Lee, Do Youn

AU - Ji, In Hye

AU - Kim, Gi Eun

AU - Kim, Hye Nam

AU - Sohn, Jeongwon

AU - Kim, Sangduk

AU - Kim, Chan Wha

PY - 2001/4/1

Y1 - 2001/4/1

N2 - β-Glucan, one of the major cell wall components of Saccharomyces cerevisiae, has been found to enhance immune functions, especially by activating macrophages. However, a major obstacle to the clinical application of β-(1→3)-glucan is its low solubility in aqueous media. In this study, soluble β-glucan, free of mannoprotein, was prepared, and its effects on TNF-α secretion and phagocytosis by macrophages were evaluated. β-Glucan was first rendered soluble from the yeast cell wall by alkaline extraction (glucan-p1). The extract contained 2.8% of protein which was subsequently removed by successive DEAE-cellulose and ConA chromatography. β-Glucan thus prepared was completely free of mannoprotein and was soluble at neutral pH (glucan-p3). The effects of β-glucan on phagocytosis and TNF-α release activity were investigated. While glucan-p1 moderately induced TNF-α secretion at 200 μg/ml (550 pg of TNF-α/5 × 105 cells), glucan-p3 markedly stimulated macrophages at 200 μg/ml (2,860 pg of TNF-α/5 × 105 cells). Furthermore, glucan-p3 stimulated phagocytosis about 20% more than glucan-p1 did. In conclusion, we purified water-soluble β-glucan which was completely devoid of mannoprotein and effectively stimulated the macrophage function, enabling it to be used as an intravenous injection for sepsis.

AB - β-Glucan, one of the major cell wall components of Saccharomyces cerevisiae, has been found to enhance immune functions, especially by activating macrophages. However, a major obstacle to the clinical application of β-(1→3)-glucan is its low solubility in aqueous media. In this study, soluble β-glucan, free of mannoprotein, was prepared, and its effects on TNF-α secretion and phagocytosis by macrophages were evaluated. β-Glucan was first rendered soluble from the yeast cell wall by alkaline extraction (glucan-p1). The extract contained 2.8% of protein which was subsequently removed by successive DEAE-cellulose and ConA chromatography. β-Glucan thus prepared was completely free of mannoprotein and was soluble at neutral pH (glucan-p3). The effects of β-glucan on phagocytosis and TNF-α release activity were investigated. While glucan-p1 moderately induced TNF-α secretion at 200 μg/ml (550 pg of TNF-α/5 × 105 cells), glucan-p3 markedly stimulated macrophages at 200 μg/ml (2,860 pg of TNF-α/5 × 105 cells). Furthermore, glucan-p3 stimulated phagocytosis about 20% more than glucan-p1 did. In conclusion, we purified water-soluble β-glucan which was completely devoid of mannoprotein and effectively stimulated the macrophage function, enabling it to be used as an intravenous injection for sepsis.

KW - β-glucan

KW - Macrophage function

KW - Mannoprotein

KW - Phagocytosis

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=0035320059&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035320059&partnerID=8YFLogxK

U2 - 10.1271/bbb.65.837

DO - 10.1271/bbb.65.837

M3 - Article

VL - 65

SP - 837

EP - 841

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 4

ER -