Purification of soluble β-glucan with immune-enhancing activity from the cell wall of yeast

Jung Nam Lee, Do Youn Lee, In Hye Ji, Gi Eun Kim, Hye Nam Kim, Jeongwon Sohn, Sangduk Kim, Chan Wha Kim

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69 Citations (Scopus)

Abstract

β-Glucan, one of the major cell wall components of Saccharomyces cerevisiae, has been found to enhance immune functions, especially by activating macrophages. However, a major obstacle to the clinical application of β-(1→3)-glucan is its low solubility in aqueous media. In this study, soluble β-glucan, free of mannoprotein, was prepared, and its effects on TNF-α secretion and phagocytosis by macrophages were evaluated. β-Glucan was first rendered soluble from the yeast cell wall by alkaline extraction (glucan-p1). The extract contained 2.8% of protein which was subsequently removed by successive DEAE-cellulose and ConA chromatography. β-Glucan thus prepared was completely free of mannoprotein and was soluble at neutral pH (glucan-p3). The effects of β-glucan on phagocytosis and TNF-α release activity were investigated. While glucan-p1 moderately induced TNF-α secretion at 200 μg/ml (550 pg of TNF-α/5 × 105 cells), glucan-p3 markedly stimulated macrophages at 200 μg/ml (2,860 pg of TNF-α/5 × 105 cells). Furthermore, glucan-p3 stimulated phagocytosis about 20% more than glucan-p1 did. In conclusion, we purified water-soluble β-glucan which was completely devoid of mannoprotein and effectively stimulated the macrophage function, enabling it to be used as an intravenous injection for sepsis.

Original languageEnglish
Pages (from-to)837-841
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number4
DOIs
Publication statusPublished - 2001 Apr 1

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Keywords

  • Macrophage function
  • Mannoprotein
  • Phagocytosis
  • TNF-α
  • β-glucan

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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