Pusillimonas harenae sp. nov., isolated from a sandy beach, and emended description of the genus pusillimonas

Moon Su Park, Youn Je Park, Ji Young Jung, Seung Hyeon Lee, Woojun Park, Kangseok Lee, Che Ok Jeon

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A Gram-stain-negative, motile bacterium with two lateral flagella, designated strain B201T, was isolated from beach sand from the Taean coast in South Korea. Cells were ovoid rods and positive for catalase and oxidase. Growth of strain B201T was observed between 15 and 45 °C (optimum, 30 °C) and between pH 5.0 and 9.0 (optimum, pH 6.0-7.5). Strain B201T contained ubiquinone Q-8 as the major isoprenoid quinone, but MK-6 was also present as a minor quinone. The major fatty acids of strain B201T were C 17:0 cyclo, C 16:0, summed feature 2 (iso-C 16:1 I/C 14:0 3-OH and/or C 12:0 ALDE), C 12:0 and C 19:0 cyclo ω8c. The major cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and three aminolipids. The genomic DNA G+C content was 53.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a phyletic lineage with Pusillimonas ginsengisoli DCY25 T within the genus Pusillimonas. Strain B201 T was most closely related to P. ginsengisoli DCY25 T and Pusillimonas soli MJ07 T with similarities of 98.6 and 97.5%, respectively. However, DNA-DNA relatedness values of strain B201 T with P. ginsengisoli DCY25 T and P. soli MJ07 T were 30.2±5.4 and 4.9±1.8%, respectively. On the basis of chemotaxonomic data and molecular properties, strain B201 T represents a novel species of the genus Pusillimonas, for which the name Pusillimonas harenae sp. nov. is proposed; the type strain is B201 T (=KACC 14927 T=JCM 16917 T). An emended description of the genus Pusillimonas is given.

Original languageEnglish
Pages (from-to)2901-2906
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number12
DOIs
Publication statusPublished - 2011 Dec 1

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aprepitant
Beaches
beaches
beach
DNA
new species
Ancitabine
Republic of Korea
Phosphatidylglycerols
Cardiolipins
Flagella
isoprenoid
Terpenes
Base Composition
phospholipid
rRNA Genes
relatedness
Catalase
Names
Phospholipids

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Pusillimonas harenae sp. nov., isolated from a sandy beach, and emended description of the genus pusillimonas. / Park, Moon Su; Park, Youn Je; Jung, Ji Young; Lee, Seung Hyeon; Park, Woojun; Lee, Kangseok; Jeon, Che Ok.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 12, 01.12.2011, p. 2901-2906.

Research output: Contribution to journalArticle

Park, Moon Su ; Park, Youn Je ; Jung, Ji Young ; Lee, Seung Hyeon ; Park, Woojun ; Lee, Kangseok ; Jeon, Che Ok. / Pusillimonas harenae sp. nov., isolated from a sandy beach, and emended description of the genus pusillimonas. In: International Journal of Systematic and Evolutionary Microbiology. 2011 ; Vol. 61, No. 12. pp. 2901-2906.
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