Putative embryonic stem cells derived from porcine cloned blastocysts using induced pluripotent stem cells as donors

Eunhye Kim, Seon Ung Hwang, Hyunju Yoo, Junchul David Yoon, Yubyeol Jeon, Hyunggee Kim, Eui Bae Jeung, Chang Kyu Lee, Sang Hwan Hyun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The establishment of porcine embryonic stem cells (ESCs) would have great impact in biomedical studies and preclinical trials through their use in genetic engineering. However, authentic porcine ESCs have not been established until now. In this study, a total of seven putative ESC lines were derived from porcine embryos of various origins, including in vitro fertilization, parthenogenetic activation, and, in particular, induced pluripotent stem (iPS) nuclear transfer (NT) from a donor cell with induced pluripotent stem cells (iPSCs). To characterize these cell lines, several assays including an assessment of intensive alkaline phosphatase activity, karyotyping, embryoid body formation, expression analysis of the pluripotency-associated markers, and the three germ layerassociated markers were performed. Based on quantitative polymerase chain reaction, the expression levels of REX1 and FGFR2 in iPS-NT lines were higher than those of cells of other origins. Additionally, only iPS-NT lines showed multiple aberrant patterns of nuclear foci elucidated by immunofluorescence staining of H3K27me3 as a marker of the state of X chromosome inactivation and a less mature form of mitochondria like naive ESCs, by transmission electron microscopy. Together, these data suggested that established putative porcine ESC lines generally exhibited a primed pluripotent state, like human ESCs. However, iPS-NT lines have especially unique characteristics distinct from other origins because they have more epigenetic instability and naive-like mitochondrial morphology than other putative ESC lines. This is the first study to establish and characterize the iPSC-derived putative ESC lines and compare them with other lines derived from different origins in pigs.

Original languageEnglish
Pages (from-to)601-616
Number of pages16
JournalTheriogenology
Volume85
Issue number4
DOIs
Publication statusPublished - 2016 Mar 1

Fingerprint

Induced Pluripotent Stem Cells
embryonic stem cells
Blastocyst
Embryonic Stem Cells
blastocyst
Swine
swine
cell lines
Cell Line
stems
Embryoid Bodies
X Chromosome Inactivation
Karyotyping
Genetic Engineering
Fertilization in Vitro
induced pluripotent stem cells
Transmission Electron Microscopy
Epigenomics
in vitro fertilization
X chromosome

Keywords

  • Embryonic stem cell
  • Induced pluripotent stem cell
  • Pig
  • Pluripotency

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Equine
  • Food Animals
  • Small Animals

Cite this

Putative embryonic stem cells derived from porcine cloned blastocysts using induced pluripotent stem cells as donors. / Kim, Eunhye; Hwang, Seon Ung; Yoo, Hyunju; Yoon, Junchul David; Jeon, Yubyeol; Kim, Hyunggee; Jeung, Eui Bae; Lee, Chang Kyu; Hyun, Sang Hwan.

In: Theriogenology, Vol. 85, No. 4, 01.03.2016, p. 601-616.

Research output: Contribution to journalArticle

Kim, Eunhye ; Hwang, Seon Ung ; Yoo, Hyunju ; Yoon, Junchul David ; Jeon, Yubyeol ; Kim, Hyunggee ; Jeung, Eui Bae ; Lee, Chang Kyu ; Hyun, Sang Hwan. / Putative embryonic stem cells derived from porcine cloned blastocysts using induced pluripotent stem cells as donors. In: Theriogenology. 2016 ; Vol. 85, No. 4. pp. 601-616.
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