Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy

Ling Xi Huang; Yue Bo He; Jaewon Kim; Amit Sharma; Qian Yong Cao; Jong Seung Kim

doi:10.1039/d1cc05500c

Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy

Ling Xi Huang, Yue Bo He, Jaewon Kim, Amit Sharma, Qian Yong Cao, Jong Seung Kim

Research output: Contribution to journal › Article › peer-review

Abstract

An indicator displacement assay, namely polymericPNPY-n/UDconsisting of a cationic polynorbornene backbone with pyridinium functional groups (PNPY-1,2,3) and an anionic uranine dye (UD) as an indicator, has been developed for highly sensitive “turn-on” fluorescence sensing of ATP. WhilePNPY-1/UDitself is non-emissive, a bright green fluorescence signal was observed in the presence of ATP [K_a= 2.17 × 10⁵M⁻¹, LOD = 5.7 nM]. The potential of a highly photostable systemPNPY-1/UDwas also validated in detecting ATP levels in live-cell imaging applications.

Original language	English
Pages (from-to)	13530-13533
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	99
DOIs	https://doi.org/10.1039/d1cc05500c
Publication status	Published - 2021 Dec 25

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy",

abstract = "An indicator displacement assay, namely polymericPNPY-n/UDconsisting of a cationic polynorbornene backbone with pyridinium functional groups (PNPY-1,2,3) and an anionic uranine dye (UD) as an indicator, has been developed for highly sensitive “turn-on” fluorescence sensing of ATP. WhilePNPY-1/UDitself is non-emissive, a bright green fluorescence signal was observed in the presence of ATP [Ka= 2.17 × 105M−1, LOD = 5.7 nM]. The potential of a highly photostable systemPNPY-1/UDwas also validated in detecting ATP levels in live-cell imaging applications.",

author = "Huang, {Ling Xi} and He, {Yue Bo} and Jaewon Kim and Amit Sharma and Cao, {Qian Yong} and Kim, {Jong Seung}",

note = "Funding Information: This work was supported by the National Nature Science Foundation of China (no. 21762028 and 22061028) and the Basic Science Research Program funded by NRF of Korea (CRI project no. 2018R1A3B1052702, NRF-2019M3E5D1A01068998, JSK). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

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doi = "10.1039/d1cc05500c",

language = "English",

volume = "57",

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journal = "Chemical Communications",

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T1 - Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy

AU - Huang, Ling Xi

AU - He, Yue Bo

AU - Kim, Jaewon

AU - Sharma, Amit

AU - Cao, Qian Yong

AU - Kim, Jong Seung

N1 - Funding Information: This work was supported by the National Nature Science Foundation of China (no. 21762028 and 22061028) and the Basic Science Research Program funded by NRF of Korea (CRI project no. 2018R1A3B1052702, NRF-2019M3E5D1A01068998, JSK). Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2021/12/25

Y1 - 2021/12/25

N2 - An indicator displacement assay, namely polymericPNPY-n/UDconsisting of a cationic polynorbornene backbone with pyridinium functional groups (PNPY-1,2,3) and an anionic uranine dye (UD) as an indicator, has been developed for highly sensitive “turn-on” fluorescence sensing of ATP. WhilePNPY-1/UDitself is non-emissive, a bright green fluorescence signal was observed in the presence of ATP [Ka= 2.17 × 105M−1, LOD = 5.7 nM]. The potential of a highly photostable systemPNPY-1/UDwas also validated in detecting ATP levels in live-cell imaging applications.

AB - An indicator displacement assay, namely polymericPNPY-n/UDconsisting of a cationic polynorbornene backbone with pyridinium functional groups (PNPY-1,2,3) and an anionic uranine dye (UD) as an indicator, has been developed for highly sensitive “turn-on” fluorescence sensing of ATP. WhilePNPY-1/UDitself is non-emissive, a bright green fluorescence signal was observed in the presence of ATP [Ka= 2.17 × 105M−1, LOD = 5.7 nM]. The potential of a highly photostable systemPNPY-1/UDwas also validated in detecting ATP levels in live-cell imaging applications.

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JO - Chemical Communications

JF - Chemical Communications

IS - 99

ER -

Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy

Abstract

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