Pyrosequencing demonstrated complex microbial communities in a membrane filtration system for a drinking water treatment plant

Soondong Kwon, Eunjeong Moon, Taek Seung Kim, Seungkwan Hong, Hee-Deung Park

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Microbial community composition in a pilot-scale microfiltration plant for drinking water treatment was investigated using high-throughput pyrosequencing technology. Sequences of 16S rRNA gene fragments were recovered from raw water, membrane tank particulate matter, and membrane biofilm, and used for taxonomic assignments, estimations of diversity, and the identification of potential pathogens. Greater bacterial diversity was observed in each sample (1,133-1,731 operational taxonomic units) than studies using conventional methods, primarily due to the large number (8,164-22,275) of sequences available for analysis and the identification of rare species. Betaproteobacteria predominated in the raw water (61.1%), while Alphaproteobacteria were predominant in the membrane tank particulate matter (42.4%) and membrane biofilm (32.8%). The bacterial community structure clearly differed for each sample at both the genus and species levels, suggesting that different environmental and growth conditions were generated during membrane filtration. Moreover, signatures of potential pathogens including Legionella, Pseudomonas, Aeromonas, and Chromobacterium were identified, and the proportions of Legionella and Chromobacterium were elevated in the membrane tank particulate matter, suggesting a potential threat to drinking water treated by membrane filtration.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalMicrobes and Environments
Volume26
Issue number2
DOIs
Publication statusPublished - 2011 Jun 14

Fingerprint

Chromobacterium
water treatment
Legionella
drinking water
microbial communities
microbial community
particulates
membrane
biofilm
microfiltration
beta-Proteobacteria
Aeromonas
pathogens
alpha-Proteobacteria
particulate matter
bacterial communities
Pseudomonas
community structure
water
ribosomal RNA

Keywords

  • Drinking water
  • Membrane
  • Microbial community
  • Pathogen
  • Pyrosequencing

ASJC Scopus subject areas

  • Plant Science
  • Soil Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Pyrosequencing demonstrated complex microbial communities in a membrane filtration system for a drinking water treatment plant. / Kwon, Soondong; Moon, Eunjeong; Kim, Taek Seung; Hong, Seungkwan; Park, Hee-Deung.

In: Microbes and Environments, Vol. 26, No. 2, 14.06.2011, p. 149-155.

Research output: Contribution to journalArticle

@article{05e267451a93469daf0419fa7a85045b,
title = "Pyrosequencing demonstrated complex microbial communities in a membrane filtration system for a drinking water treatment plant",
abstract = "Microbial community composition in a pilot-scale microfiltration plant for drinking water treatment was investigated using high-throughput pyrosequencing technology. Sequences of 16S rRNA gene fragments were recovered from raw water, membrane tank particulate matter, and membrane biofilm, and used for taxonomic assignments, estimations of diversity, and the identification of potential pathogens. Greater bacterial diversity was observed in each sample (1,133-1,731 operational taxonomic units) than studies using conventional methods, primarily due to the large number (8,164-22,275) of sequences available for analysis and the identification of rare species. Betaproteobacteria predominated in the raw water (61.1{\%}), while Alphaproteobacteria were predominant in the membrane tank particulate matter (42.4{\%}) and membrane biofilm (32.8{\%}). The bacterial community structure clearly differed for each sample at both the genus and species levels, suggesting that different environmental and growth conditions were generated during membrane filtration. Moreover, signatures of potential pathogens including Legionella, Pseudomonas, Aeromonas, and Chromobacterium were identified, and the proportions of Legionella and Chromobacterium were elevated in the membrane tank particulate matter, suggesting a potential threat to drinking water treated by membrane filtration.",
keywords = "Drinking water, Membrane, Microbial community, Pathogen, Pyrosequencing",
author = "Soondong Kwon and Eunjeong Moon and Kim, {Taek Seung} and Seungkwan Hong and Hee-Deung Park",
year = "2011",
month = "6",
day = "14",
doi = "10.1264/jsme2.ME10205",
language = "English",
volume = "26",
pages = "149--155",
journal = "Microbes and Environments",
issn = "1342-6311",
publisher = "Japanese Society Of Microbial Ecology",
number = "2",

}

TY - JOUR

T1 - Pyrosequencing demonstrated complex microbial communities in a membrane filtration system for a drinking water treatment plant

AU - Kwon, Soondong

AU - Moon, Eunjeong

AU - Kim, Taek Seung

AU - Hong, Seungkwan

AU - Park, Hee-Deung

PY - 2011/6/14

Y1 - 2011/6/14

N2 - Microbial community composition in a pilot-scale microfiltration plant for drinking water treatment was investigated using high-throughput pyrosequencing technology. Sequences of 16S rRNA gene fragments were recovered from raw water, membrane tank particulate matter, and membrane biofilm, and used for taxonomic assignments, estimations of diversity, and the identification of potential pathogens. Greater bacterial diversity was observed in each sample (1,133-1,731 operational taxonomic units) than studies using conventional methods, primarily due to the large number (8,164-22,275) of sequences available for analysis and the identification of rare species. Betaproteobacteria predominated in the raw water (61.1%), while Alphaproteobacteria were predominant in the membrane tank particulate matter (42.4%) and membrane biofilm (32.8%). The bacterial community structure clearly differed for each sample at both the genus and species levels, suggesting that different environmental and growth conditions were generated during membrane filtration. Moreover, signatures of potential pathogens including Legionella, Pseudomonas, Aeromonas, and Chromobacterium were identified, and the proportions of Legionella and Chromobacterium were elevated in the membrane tank particulate matter, suggesting a potential threat to drinking water treated by membrane filtration.

AB - Microbial community composition in a pilot-scale microfiltration plant for drinking water treatment was investigated using high-throughput pyrosequencing technology. Sequences of 16S rRNA gene fragments were recovered from raw water, membrane tank particulate matter, and membrane biofilm, and used for taxonomic assignments, estimations of diversity, and the identification of potential pathogens. Greater bacterial diversity was observed in each sample (1,133-1,731 operational taxonomic units) than studies using conventional methods, primarily due to the large number (8,164-22,275) of sequences available for analysis and the identification of rare species. Betaproteobacteria predominated in the raw water (61.1%), while Alphaproteobacteria were predominant in the membrane tank particulate matter (42.4%) and membrane biofilm (32.8%). The bacterial community structure clearly differed for each sample at both the genus and species levels, suggesting that different environmental and growth conditions were generated during membrane filtration. Moreover, signatures of potential pathogens including Legionella, Pseudomonas, Aeromonas, and Chromobacterium were identified, and the proportions of Legionella and Chromobacterium were elevated in the membrane tank particulate matter, suggesting a potential threat to drinking water treated by membrane filtration.

KW - Drinking water

KW - Membrane

KW - Microbial community

KW - Pathogen

KW - Pyrosequencing

UR - http://www.scopus.com/inward/record.url?scp=79958242142&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958242142&partnerID=8YFLogxK

U2 - 10.1264/jsme2.ME10205

DO - 10.1264/jsme2.ME10205

M3 - Article

C2 - 21502735

AN - SCOPUS:79958242142

VL - 26

SP - 149

EP - 155

JO - Microbes and Environments

JF - Microbes and Environments

SN - 1342-6311

IS - 2

ER -