Quantification of Alaska pollock surimi in prepared crabstick by competitive ELISA using a myosin light chain 1 specific peptide

Zachary H. Reed, Jae W. Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) was developed for quantification of Alaska pollock (AP) surimi in crabsticks. Identification of fish species is complicated by processing, cooking, and additional ingredients. ELISA is a powerful tool for identification and quantification of fish species. Polyclonal antibodies were raised in rabbits against a 15-amino-acid peptide (Ala-Pro-Lys-Lys-Asp-Val-Lys-Ala-Pro-Ala-Ala-Ala-Ala-Lys-Lys) determined from the myosin light chain 1 (MLC 1) of AP. Immunoblotting showed the anti-pep-AP antibody had no significant cross-reactivity with protein additives. However, cross-reactivity of the MLC 1 from Pacific whiting, and threadfin bream surimi was observed. MLC 1 was purified from AP surimi and used as the coating protein in the competitive ELISA. MLC 1 was serially diluted and had a R2 of 0.9845 following a logarithmic curve. All estimations of AP surimi were within 9% of the actual value. Inter-assay coefficients of variance ranged from 4.2% to 4.9%.

Original languageEnglish
Pages (from-to)196-201
Number of pages6
JournalFood Chemistry
Volume123
Issue number1
DOIs
Publication statusPublished - 2010 Nov 1

Keywords

  • Alaska pollock
  • Competitive ELISA
  • Crabstick
  • Peptide
  • Surimi

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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