Quantification of amyloid-β secretion in isolated optofluidic chamber array

Liz Y. Wu, Yeonho Choi, Soon Gweon Hong, Helen Wu, Megan Dueck, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Amyloid-β (A β) secretion plays a critical role in the pathogenesis of Alzheimer's disease (AD) and intercellular neuronal communication is potentially an important factor influencing A β secretion levels. However, there is no existing system that can isolate a specific number of neuronal cells, encapsulate their A β secretions, and measure the dynamics of A β secretion directly to investigate how the number and density of cells affects the secretion activity. We have designed an optofluidic system to isolate one or more neuronal cells in nanoliter-volume culture chambers, where A β secretions are confined and detected. Since surface enhanced Raman scattering (SERS)-based detection is ideal for real-time and label-free A β quantification, 2D nanocrescent SERS array is integrated on the bottom floor of the cell culture chambers for A β secretion detection. This system enables, for the first time, the systematic characterization of A β secretion for different cell population densities.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages6-8
Number of pages3
Publication statusPublished - 2008 Jan 1
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Wu, L. Y., Choi, Y., Hong, S. G., Wu, H., Dueck, M., & Lee, L. P. (2008). Quantification of amyloid-β secretion in isolated optofluidic chamber array. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 6-8). Chemical and Biological Microsystems Society.