TY - JOUR
T1 - Quantification of focal adhesion dynamics of cell movement based on cell-induced collagen matrix deformation using second-harmonic generation microscopy
AU - Kang, Yong Guk
AU - Jang, Hwanseok
AU - Yang, Taeseok Daniel
AU - Notbohm, Jacob
AU - Choi, Youngwoon
AU - Park, Yongdoo
AU - Kim, Beop Min
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (Nos. 2014R1A2A1A11051879, 2017R1A6A3A11031083, and 2015H1A2A1030048) and the Ministry of Health and Welfare Grant (No. HI13C1501) funded by the Korean government.
Publisher Copyright:
© The Authors.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Mechanical interactions of living cells with the surrounding environment via focal adhesion (FA) in three dimensions (3-D) play a key role in dynamic biological events, such as tissue regeneration, wound healing, and cancer invasion. Recently, several methods for observing 3-D cell-extracellular matrix (ECM) interactions have been reported, lacking solid and quantitative analysis on the dynamics of the physical interaction between the cell and the ECM. We measured the submicron displacements of ECM deformation in 3-D due to protrusion-retraction dynamics during cell migration, using second-harmonic generation without labeling the matrix structures. We then quantitatively analyzed the mechanical deformation between the ECM and the cells based on spatiotemporal volumetric correlations. The greatest deformations within the collagen matrix were found to occur at sites of colocalization of the FA site-related proteins vinculin and actin, which confirms that FA sites play a critical role in living cells within the ECM as a point for adhesion, traction, and migration. We believe that this modality can be used in studies of cell-ECM interaction during angiogenesis, wound healing, and metastasis.
AB - Mechanical interactions of living cells with the surrounding environment via focal adhesion (FA) in three dimensions (3-D) play a key role in dynamic biological events, such as tissue regeneration, wound healing, and cancer invasion. Recently, several methods for observing 3-D cell-extracellular matrix (ECM) interactions have been reported, lacking solid and quantitative analysis on the dynamics of the physical interaction between the cell and the ECM. We measured the submicron displacements of ECM deformation in 3-D due to protrusion-retraction dynamics during cell migration, using second-harmonic generation without labeling the matrix structures. We then quantitatively analyzed the mechanical deformation between the ECM and the cells based on spatiotemporal volumetric correlations. The greatest deformations within the collagen matrix were found to occur at sites of colocalization of the FA site-related proteins vinculin and actin, which confirms that FA sites play a critical role in living cells within the ECM as a point for adhesion, traction, and migration. We believe that this modality can be used in studies of cell-ECM interaction during angiogenesis, wound healing, and metastasis.
KW - cell migration
KW - digital volume correlation
KW - focal adhesion
KW - matrix deformation
KW - second-harmonic generation
UR - http://www.scopus.com/inward/record.url?scp=85048797842&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.23.6.065001
DO - 10.1117/1.JBO.23.6.065001
M3 - Article
C2 - 29886577
AN - SCOPUS:85048797842
VL - 23
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 6
M1 - 065001
ER -