Quantification of nucleolar organizer regions in skin tumors

Yul Lee Sung Yul Lee, Hyeok Kim Chong Hyeok Kim, Chil Hwan Oh

Research output: Contribution to journalArticle

Abstract

Background: Nucleolar organizer regions (NORs) have recently attracted much attention because of claims that their frequency within nuclei is significantly higher in malignant cells than in normal, reactive, or benign neoplastic cells. Objective: The purpose of this paper is to analyze a method allowing selection of the best morphometric criterion for quantifying AgNOR proteins under conventional observation conditions by light microscopy. Method: We tried to investigate the various parameters including NORs counting in cutaneous tumors using image analysis system. Results: There were significant differences in mean nucleus area per a AgNOR, nucleus area between the benign and potentially malignant group. But the conventional counting of AgNORs is not able to differentiate between the two groups. We could discriminate squamous cell carcinoma from Bowen's disease using parameters of mean ratio of AgNORs area per nucleus area, mean ratio of greatest AgNORs area per nucleus area, coefficient of variation (CV) of nucleus area, and mean area of largest AgNORs. In squamous cell carcinoma and keratoacanthoma, CV of nucleus area has shown a significant difference. Conclusion: Study of AgNORs using image analysis system is a useful tool for the diagnosis of cutaneous tumors.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalAnnals of Dermatology
Volume6
Issue number2
Publication statusPublished - 1994 Jan 1
Externally publishedYes

Fingerprint

Nucleolus Organizer Region
Skin
Neoplasms
Squamous Cell Carcinoma
Keratoacanthoma
Bowen's Disease
nucleolar organizer region associated proteins
Microscopy
Observation
Light

Keywords

  • Image analysis system
  • Skin tumor

ASJC Scopus subject areas

  • Dermatology

Cite this

Sung Yul Lee, Y. L., Chong Hyeok Kim, H. K., & Oh, C. H. (1994). Quantification of nucleolar organizer regions in skin tumors. Annals of Dermatology, 6(2), 140-145.

Quantification of nucleolar organizer regions in skin tumors. / Sung Yul Lee, Yul Lee; Chong Hyeok Kim, Hyeok Kim; Oh, Chil Hwan.

In: Annals of Dermatology, Vol. 6, No. 2, 01.01.1994, p. 140-145.

Research output: Contribution to journalArticle

Sung Yul Lee, YL, Chong Hyeok Kim, HK & Oh, CH 1994, 'Quantification of nucleolar organizer regions in skin tumors', Annals of Dermatology, vol. 6, no. 2, pp. 140-145.
Sung Yul Lee YL, Chong Hyeok Kim HK, Oh CH. Quantification of nucleolar organizer regions in skin tumors. Annals of Dermatology. 1994 Jan 1;6(2):140-145.
Sung Yul Lee, Yul Lee ; Chong Hyeok Kim, Hyeok Kim ; Oh, Chil Hwan. / Quantification of nucleolar organizer regions in skin tumors. In: Annals of Dermatology. 1994 ; Vol. 6, No. 2. pp. 140-145.
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