Quantifying L-ascorbic acid-driven inhibitory effect on amyloid fibrillation

Wonseok Lee, Insu Kim, Sang Won Lee, Hyungbeen Lee, Gyudo Lee, Sangsig Kim, Sang Woo Lee, Dae Sung Yoon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ascorbic acid, which is widely used as a therapeutic agent for various disorders (e.g. chronic diseases and cancers), has potential therapeutic roles for neurodegenerative disease such as Huntington’s, Parkinson’s and Alzheimer’s diseases. The ability to impede amyloid fibril formation has a great demand on developing clinical medicine with respect to successfully preventing neurodegenerative diseases. Here, we report that L-ascorbic acid inhibits β-lactoglobulin amyloid formation in vitro. For quantitative characterization of the inhibitory effect of L-ascorbic acid on fibrillation, we performed high-resolution atomic force microscopy and thioflavin T fluorescence assay. Fourier transform infrared spectra indicated secondary structure differences of fibrils formed with and without ascorbic acid. These results suggest great potential of ascorbic acid for use in the prevention or treatment of amyloidogenic diseases.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalMacromolecular Research
DOIs
Publication statusAccepted/In press - 2016 Sep 8

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Keywords

  • atomic force microscopy
  • inhibition effect
  • L-ascorbic acid
  • thioflavin T fluorescence assay
  • β-lactoglobulin amyloid fibril

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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