TY - GEN
T1 - Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device
AU - Choi, H. I.
AU - Kim, J. Y.H.
AU - Choi, J. W.
AU - Sim, S. J.
N1 - Funding Information:
This study was supported by Grant No. 2014M1A8A1049278 from Korea CCS R&D Center of the NRF funded by the Ministry of Science, ICT, and Future Planning of Korea, the National Research Foundation of Korea (NRF) (Grant No. NRF-2013R1A2A1A01015644/2010-0027955), the Korea Institute of Energy Technology Evaluation and Planning and Ministry of Trade, Industry and Energy of in "Energy Efficiency and Resources Technology R&D" project Korea (Grant No. 20152010201900), and University-Institute Cooperation Program (2013).
PY - 2016
Y1 - 2016
N2 - Microalgae are capable of using bicarbonate (HCO3-) directly as an inorganic carbon species. Despite the importance of HCO3- in aquatic photosynthesis, little is known about the chemotaxis of microalgae towards HCO3-. Here, we demonstrated the chemotaxis of a model alga, Chlamydomonas reinhardtii, to HCO3- using an agarose gel-based zero-flow microfluidic device for generating a stable concentration gradient. We found C. reinhardtii showed the strongest chemotaxis towards HCO3- at the concentration of 26mM in the microfluidic system. We also observed that the various physiological conditions, such as circadian rhythm, mutation, and treatment of inhibitor have influence on the chemotaxis.
AB - Microalgae are capable of using bicarbonate (HCO3-) directly as an inorganic carbon species. Despite the importance of HCO3- in aquatic photosynthesis, little is known about the chemotaxis of microalgae towards HCO3-. Here, we demonstrated the chemotaxis of a model alga, Chlamydomonas reinhardtii, to HCO3- using an agarose gel-based zero-flow microfluidic device for generating a stable concentration gradient. We found C. reinhardtii showed the strongest chemotaxis towards HCO3- at the concentration of 26mM in the microfluidic system. We also observed that the various physiological conditions, such as circadian rhythm, mutation, and treatment of inhibitor have influence on the chemotaxis.
KW - Bicarbonate
KW - Chemotaxis
KW - Diffusion
KW - Microalgae
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M3 - Conference contribution
AN - SCOPUS:85014226816
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 1689
EP - 1690
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -