Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device

H. I. Choi, J. Y H Kim, J. W. Choi, Sang Jun Sim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microalgae are capable of using bicarbonate (HCO3 -) directly as an inorganic carbon species. Despite the importance of HCO3 - in aquatic photosynthesis, little is known about the chemotaxis of microalgae towards HCO3 -. Here, we demonstrated the chemotaxis of a model alga, Chlamydomonas reinhardtii, to HCO3 - using an agarose gel-based zero-flow microfluidic device for generating a stable concentration gradient. We found C. reinhardtii showed the strongest chemotaxis towards HCO3 - at the concentration of 26mM in the microfluidic system. We also observed that the various physiological conditions, such as circadian rhythm, mutation, and treatment of inhibitor have influence on the chemotaxis.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1689-1690
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

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Keywords

  • Bicarbonate
  • Chemotaxis
  • Diffusion
  • Microalgae

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Choi, H. I., Kim, J. Y. H., Choi, J. W., & Sim, S. J. (2016). Quantitative analysis of the chemotactic response of a motile microalga, Chlamydomonas reinhardtii, towards bicarbonate using flow-free microfluidic device. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1689-1690). Chemical and Biological Microsystems Society.