Quantitative analysis of the interaction between the envelope protein domains and the core protein of human hepatitis B virus

Kyoung J. Choi, Chun W. Lim, Moon Young Yoon, Byung-Yoon Ahn, Yeon Gyu Yu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Interaction between preformed nucleocapsids and viral envelope proteins is critical for the assembly of virus particles in infected cells. The pre-S1 and pre-S2 and cytosolic regions of the human hepatitis B virus envelope protein had been implicated in the interaction with the core protein of nucleocapsids. The binding affinities of specific subdomains of the envelope protein to the core protein were quantitatively measured by both ELISA and BIAcore assay. While a marginal binding was detected with the pre-S1 or pre-S2, the core protein showed high affinities to pre-S with apparent dissociation constants (K Dapp) of 7.3±0.9 and 8.2±0.4μM by ELISA and BIAcore assay, respectively. The circular dichroism analysis suggested that conformational change occurs in pre-S through interaction with core protein. These results substantiate the importance of specific envelope domains in virion assembly, and demonstrate that the interaction between viral proteins can be quantitatively measured in vitro.

Original languageEnglish
Pages (from-to)959-966
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume319
Issue number3
DOIs
Publication statusPublished - 2004 Jul 2

Fingerprint

Viruses
Hepatitis B virus
Viral Envelope Proteins
Chemical analysis
Virion
Assays
Proteins
Enzyme-Linked Immunosorbent Assay
Nucleocapsid Proteins
Nucleocapsid
Viral Proteins
Circular Dichroism
human core protein
Protein Domains

Keywords

  • Core protein
  • Hepatitis B virus
  • Interaction
  • Pre-S domains
  • Surface protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Quantitative analysis of the interaction between the envelope protein domains and the core protein of human hepatitis B virus. / Choi, Kyoung J.; Lim, Chun W.; Yoon, Moon Young; Ahn, Byung-Yoon; Yu, Yeon Gyu.

In: Biochemical and Biophysical Research Communications, Vol. 319, No. 3, 02.07.2004, p. 959-966.

Research output: Contribution to journalArticle

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