TY - JOUR
T1 - Quantitative Analysis on Cellular Uptake of Clustered Ferrite Magnetic Nanoparticles
AU - Kim, Yu Jin
AU - Park, Bum Chul
AU - Choi, Young Soo
AU - Ko, Min Jun
AU - Kim, Young Keun
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Abstract: Due to their ability to be internalized into cells by endocytosis through cell membranes, the application of nanoparticles in therapeutic and diagnostic fields has received much interest. In particular, ferrite magnetic nanoparticles (MNPs) are widely used as reagents for medical care, including in vitro magnetic separation, T2-weighted magnetic resonance imaging, magnetic hyperthermia therapy, and as drug delivery systems. However, little is known about the quantitative analysis of the cellular uptake of MNPs by the interaction of particle surfaces with biomolecules. Here, we quantitatively analyze the intracellular uptakes of 30 nm Fe- and Mn-ferrite MNPs. We confirm that the magnetic properties of MNPs change according to their microstructure and quantitatively analyze nanoparticle internalization in breast cell lines (MCF10A, MCF7, and MDA-MB-231) by measuring the magnetic moment using a vibrating sample magnetometer. Finally, we examine the effect of nanoparticle microstructure on cellular uptake in terms of the interaction between the nanoparticles and biomolecules. Graphical Abstract: [Figure not available: see fulltext.].
AB - Abstract: Due to their ability to be internalized into cells by endocytosis through cell membranes, the application of nanoparticles in therapeutic and diagnostic fields has received much interest. In particular, ferrite magnetic nanoparticles (MNPs) are widely used as reagents for medical care, including in vitro magnetic separation, T2-weighted magnetic resonance imaging, magnetic hyperthermia therapy, and as drug delivery systems. However, little is known about the quantitative analysis of the cellular uptake of MNPs by the interaction of particle surfaces with biomolecules. Here, we quantitatively analyze the intracellular uptakes of 30 nm Fe- and Mn-ferrite MNPs. We confirm that the magnetic properties of MNPs change according to their microstructure and quantitatively analyze nanoparticle internalization in breast cell lines (MCF10A, MCF7, and MDA-MB-231) by measuring the magnetic moment using a vibrating sample magnetometer. Finally, we examine the effect of nanoparticle microstructure on cellular uptake in terms of the interaction between the nanoparticles and biomolecules. Graphical Abstract: [Figure not available: see fulltext.].
KW - Biomolecule
KW - Cellular uptake
KW - Ferrite
KW - Magnetic nanoparticle
KW - Quantitative analysis
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U2 - 10.1007/s13391-019-00141-y
DO - 10.1007/s13391-019-00141-y
M3 - Article
AN - SCOPUS:85066083297
VL - 15
SP - 471
EP - 480
JO - Electronic Materials Letters
JF - Electronic Materials Letters
SN - 1738-8090
IS - 4
ER -