Quantitative Analysis on Cellular Uptake of Clustered Ferrite Magnetic Nanoparticles

Yu Jin Kim, Bum Chul Park, Young Soo Choi, Min Jun Ko, Young Keun Kim

Research output: Contribution to journalArticle

Abstract

Abstract: Due to their ability to be internalized into cells by endocytosis through cell membranes, the application of nanoparticles in therapeutic and diagnostic fields has received much interest. In particular, ferrite magnetic nanoparticles (MNPs) are widely used as reagents for medical care, including in vitro magnetic separation, T2-weighted magnetic resonance imaging, magnetic hyperthermia therapy, and as drug delivery systems. However, little is known about the quantitative analysis of the cellular uptake of MNPs by the interaction of particle surfaces with biomolecules. Here, we quantitatively analyze the intracellular uptakes of 30 nm Fe- and Mn-ferrite MNPs. We confirm that the magnetic properties of MNPs change according to their microstructure and quantitatively analyze nanoparticle internalization in breast cell lines (MCF10A, MCF7, and MDA-MB-231) by measuring the magnetic moment using a vibrating sample magnetometer. Finally, we examine the effect of nanoparticle microstructure on cellular uptake in terms of the interaction between the nanoparticles and biomolecules. Graphical Abstract: [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)471-480
Number of pages10
JournalElectronic Materials Letters
Volume15
Issue number4
DOIs
Publication statusPublished - 2019 Jul 1

Keywords

  • Biomolecule
  • Cellular uptake
  • Ferrite
  • Magnetic nanoparticle
  • Quantitative analysis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

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