Quantitative Analysis on Cellular Uptake of Clustered Ferrite Magnetic Nanoparticles

Yu Jin Kim; Bum Chul Park; Young Soo Choi; Min Jun Ko; Young Keun Kim

doi:10.1007/s13391-019-00141-y

Quantitative Analysis on Cellular Uptake of Clustered Ferrite Magnetic Nanoparticles

Yu Jin Kim, Bum Chul Park, Young Soo Choi, Min Jun Ko, Young Keun Kim

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Abstract: Due to their ability to be internalized into cells by endocytosis through cell membranes, the application of nanoparticles in therapeutic and diagnostic fields has received much interest. In particular, ferrite magnetic nanoparticles (MNPs) are widely used as reagents for medical care, including in vitro magnetic separation, T2-weighted magnetic resonance imaging, magnetic hyperthermia therapy, and as drug delivery systems. However, little is known about the quantitative analysis of the cellular uptake of MNPs by the interaction of particle surfaces with biomolecules. Here, we quantitatively analyze the intracellular uptakes of 30 nm Fe- and Mn-ferrite MNPs. We confirm that the magnetic properties of MNPs change according to their microstructure and quantitatively analyze nanoparticle internalization in breast cell lines (MCF10A, MCF7, and MDA-MB-231) by measuring the magnetic moment using a vibrating sample magnetometer. Finally, we examine the effect of nanoparticle microstructure on cellular uptake in terms of the interaction between the nanoparticles and biomolecules. Graphical Abstract: [Figure not available: see fulltext.].

Original language	English
Pages (from-to)	471-480
Number of pages	10
Journal	Electronic Materials Letters
Volume	15
Issue number	4
DOIs	https://doi.org/10.1007/s13391-019-00141-y
Publication status	Published - 2019 Jul 1

Keywords

Biomolecule
Cellular uptake
Ferrite
Magnetic nanoparticle
Quantitative analysis

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials

Access to Document

10.1007/s13391-019-00141-y

Cite this

@article{91bdc4fafb8b454ea05d5288c0e66885,

title = "Quantitative Analysis on Cellular Uptake of Clustered Ferrite Magnetic Nanoparticles",

abstract = "Abstract: Due to their ability to be internalized into cells by endocytosis through cell membranes, the application of nanoparticles in therapeutic and diagnostic fields has received much interest. In particular, ferrite magnetic nanoparticles (MNPs) are widely used as reagents for medical care, including in vitro magnetic separation, T2-weighted magnetic resonance imaging, magnetic hyperthermia therapy, and as drug delivery systems. However, little is known about the quantitative analysis of the cellular uptake of MNPs by the interaction of particle surfaces with biomolecules. Here, we quantitatively analyze the intracellular uptakes of 30 nm Fe- and Mn-ferrite MNPs. We confirm that the magnetic properties of MNPs change according to their microstructure and quantitatively analyze nanoparticle internalization in breast cell lines (MCF10A, MCF7, and MDA-MB-231) by measuring the magnetic moment using a vibrating sample magnetometer. Finally, we examine the effect of nanoparticle microstructure on cellular uptake in terms of the interaction between the nanoparticles and biomolecules. Graphical Abstract: [Figure not available: see fulltext.].",

keywords = "Biomolecule, Cellular uptake, Ferrite, Magnetic nanoparticle, Quantitative analysis",

author = "Kim, {Yu Jin} and Park, {Bum Chul} and Choi, {Young Soo} and Ko, {Min Jun} and Kim, {Young Keun}",

note = "Funding Information: This work was supported through the National Research Foundation of Korea funded by the Ministry of Science and ICT (2014M3A7B4052193, 2019R1A2C3006587), and by the Ministry of Trade, Industry and Energy of Korea under Industrial Technology Innovation Program (10080408). Y.J.K. received financial support from the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2017R1D1A1B03036100). Funding Information: Acknowledgements This work was supported through the National Research Foundation of Korea funded by the Ministry of Science and ICT (2014M3A7B4052193, 2019R1A2C3006587), and by the Ministry of Trade, Industry and Energy of Korea under Industrial Technology Innovation Program (10080408). Y.J.K. received financial support from the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2017R1D1A1B03036100). Publisher Copyright: {\textcopyright} 2019, The Korean Institute of Metals and Materials.",

year = "2019",

month = jul,

day = "1",

doi = "10.1007/s13391-019-00141-y",

language = "English",

volume = "15",

pages = "471--480",

journal = "Electronic Materials Letters",

issn = "1738-8090",

publisher = "Springer Netherlands",

number = "4",

}

TY - JOUR

T1 - Quantitative Analysis on Cellular Uptake of Clustered Ferrite Magnetic Nanoparticles

AU - Kim, Yu Jin

AU - Park, Bum Chul

AU - Choi, Young Soo

AU - Ko, Min Jun

AU - Kim, Young Keun

N1 - Funding Information: This work was supported through the National Research Foundation of Korea funded by the Ministry of Science and ICT (2014M3A7B4052193, 2019R1A2C3006587), and by the Ministry of Trade, Industry and Energy of Korea under Industrial Technology Innovation Program (10080408). Y.J.K. received financial support from the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2017R1D1A1B03036100). Funding Information: Acknowledgements This work was supported through the National Research Foundation of Korea funded by the Ministry of Science and ICT (2014M3A7B4052193, 2019R1A2C3006587), and by the Ministry of Trade, Industry and Energy of Korea under Industrial Technology Innovation Program (10080408). Y.J.K. received financial support from the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2017R1D1A1B03036100). Publisher Copyright: © 2019, The Korean Institute of Metals and Materials.

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Abstract: Due to their ability to be internalized into cells by endocytosis through cell membranes, the application of nanoparticles in therapeutic and diagnostic fields has received much interest. In particular, ferrite magnetic nanoparticles (MNPs) are widely used as reagents for medical care, including in vitro magnetic separation, T2-weighted magnetic resonance imaging, magnetic hyperthermia therapy, and as drug delivery systems. However, little is known about the quantitative analysis of the cellular uptake of MNPs by the interaction of particle surfaces with biomolecules. Here, we quantitatively analyze the intracellular uptakes of 30 nm Fe- and Mn-ferrite MNPs. We confirm that the magnetic properties of MNPs change according to their microstructure and quantitatively analyze nanoparticle internalization in breast cell lines (MCF10A, MCF7, and MDA-MB-231) by measuring the magnetic moment using a vibrating sample magnetometer. Finally, we examine the effect of nanoparticle microstructure on cellular uptake in terms of the interaction between the nanoparticles and biomolecules. Graphical Abstract: [Figure not available: see fulltext.].

AB - Abstract: Due to their ability to be internalized into cells by endocytosis through cell membranes, the application of nanoparticles in therapeutic and diagnostic fields has received much interest. In particular, ferrite magnetic nanoparticles (MNPs) are widely used as reagents for medical care, including in vitro magnetic separation, T2-weighted magnetic resonance imaging, magnetic hyperthermia therapy, and as drug delivery systems. However, little is known about the quantitative analysis of the cellular uptake of MNPs by the interaction of particle surfaces with biomolecules. Here, we quantitatively analyze the intracellular uptakes of 30 nm Fe- and Mn-ferrite MNPs. We confirm that the magnetic properties of MNPs change according to their microstructure and quantitatively analyze nanoparticle internalization in breast cell lines (MCF10A, MCF7, and MDA-MB-231) by measuring the magnetic moment using a vibrating sample magnetometer. Finally, we examine the effect of nanoparticle microstructure on cellular uptake in terms of the interaction between the nanoparticles and biomolecules. Graphical Abstract: [Figure not available: see fulltext.].

KW - Biomolecule

KW - Cellular uptake

KW - Ferrite

KW - Magnetic nanoparticle

KW - Quantitative analysis

UR - http://www.scopus.com/inward/record.url?scp=85066083297&partnerID=8YFLogxK

U2 - 10.1007/s13391-019-00141-y

DO - 10.1007/s13391-019-00141-y

M3 - Article

AN - SCOPUS:85066083297

VL - 15

SP - 471

EP - 480

JO - Electronic Materials Letters

JF - Electronic Materials Letters

SN - 1738-8090

IS - 4

ER -

Quantitative Analysis on Cellular Uptake of Clustered Ferrite Magnetic Nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this