Quantitative characterization for dielectrophoretic behavior of biological cells using optical tweezers

In Soo Park; Se Hee Park; Sang Woo Lee; Dae Sung Yoon; Beop Min Kim

doi:10.1063/1.4862746

Quantitative characterization for dielectrophoretic behavior of biological cells using optical tweezers

In Soo Park, Se Hee Park, Sang Woo Lee, Dae Sung Yoon, Beop Min Kim

School of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We report a method to precisely quantify dielectrophoretic (DEP) forces and cutoff frequencies (f_c) of viable and nonviable yeast cells. The method consists of a two-step process in which generated DEP forces act upon a cell through a micro-electrode device, followed by direct measurement of DEP forces using optical tweezers. DEP behaviors of viable and nonviable yeast cells are monitored as a function of AC frequency. We believe that the proposed method can be used as a powerful platform for cell-based assays to characterize the DEP behavior of various cell types including cancer and normal cells.

Original language	English
Article number	053701
Journal	Applied Physics Letters
Volume	104
Issue number	5
DOIs	https://doi.org/10.1063/1.4862746
Publication status	Published - 2014 Feb 3

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/1.4862746

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abstract = "We report a method to precisely quantify dielectrophoretic (DEP) forces and cutoff frequencies (fc) of viable and nonviable yeast cells. The method consists of a two-step process in which generated DEP forces act upon a cell through a micro-electrode device, followed by direct measurement of DEP forces using optical tweezers. DEP behaviors of viable and nonviable yeast cells are monitored as a function of AC frequency. We believe that the proposed method can be used as a powerful platform for cell-based assays to characterize the DEP behavior of various cell types including cancer and normal cells.",

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AU - Park, In Soo

AU - Park, Se Hee

AU - Lee, Sang Woo

AU - Yoon, Dae Sung

AU - Kim, Beop Min

PY - 2014/2/3

Y1 - 2014/2/3

N2 - We report a method to precisely quantify dielectrophoretic (DEP) forces and cutoff frequencies (fc) of viable and nonviable yeast cells. The method consists of a two-step process in which generated DEP forces act upon a cell through a micro-electrode device, followed by direct measurement of DEP forces using optical tweezers. DEP behaviors of viable and nonviable yeast cells are monitored as a function of AC frequency. We believe that the proposed method can be used as a powerful platform for cell-based assays to characterize the DEP behavior of various cell types including cancer and normal cells.

AB - We report a method to precisely quantify dielectrophoretic (DEP) forces and cutoff frequencies (fc) of viable and nonviable yeast cells. The method consists of a two-step process in which generated DEP forces act upon a cell through a micro-electrode device, followed by direct measurement of DEP forces using optical tweezers. DEP behaviors of viable and nonviable yeast cells are monitored as a function of AC frequency. We believe that the proposed method can be used as a powerful platform for cell-based assays to characterize the DEP behavior of various cell types including cancer and normal cells.

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Quantitative characterization for dielectrophoretic behavior of biological cells using optical tweezers

Abstract

ASJC Scopus subject areas

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