Quantitative detection of DNA by autocatalytic enlargement of hybridized gold nanoprobes

Zongrui Zhan, Cuong Cao, Sang Jun Sim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Quantitative detection of specific viral DNA has become a pressing issue for the earlier clinical diagnosis of viral infectious diseases. Therefore, in this paper, we report a simple, sensitive, and inexpensive quantitative approach for DNA detection based on the autocatalytic Au deposition of gold nanoprobes via the surface reduction of AuCl4 - to Au0 on their surface in the presence of ascorbic acid (AA) and cetyltrimethylammonium bromide (CTAB). On this basis, signal enhancements in the absorbance intensity and kinetic behavior of gold enlargement in the aqueous phase have been well investigated and explained for the selection of analytical parameters. To achieve high sensitivity, magnetic particles conjugated with capture probes (PMPs) were employed for the collection of gold nanoprobes. After denaturated by ion a pH 11 solution, the amplified signals of gold nanoprobes, which is proportional to the concentration of the target DNA, could easily be confirmed by a UV-vis scanning spectrophotometer. Limit of detection could be obtained as low as 1.0fM by this simple method.

Original languageEnglish
Pages (from-to)511-516
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number2
DOIs
Publication statusPublished - 2010 Oct 1
Externally publishedYes

Fingerprint

Nanoprobes
Gold
DNA
Ascorbic acid
Spectrophotometers
Viral DNA
Virus Diseases
Ascorbic Acid
Communicable Diseases
Limit of Detection
Early Diagnosis
Ions
Scanning
Kinetics

Keywords

  • Aqueous phase
  • Ascorbic acid
  • Autocatalytic enlargement
  • DNA quantitative detection
  • Gold nanoprobe

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Quantitative detection of DNA by autocatalytic enlargement of hybridized gold nanoprobes. / Zhan, Zongrui; Cao, Cuong; Sim, Sang Jun.

In: Biosensors and Bioelectronics, Vol. 26, No. 2, 01.10.2010, p. 511-516.

Research output: Contribution to journalArticle

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