Quantitative detection of glyphosate by simultaneous analysis of UV spectroscopy and fluorescence using DNA-labeled gold nanoparticles

Hee Uk Lee, Hyun Yong Shin, Jin Young Lee, Yoon Seok Song, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A sandwich-type immunosensor composed of antigen-double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement of fluorescence intensity and quantitative analysis of single-stranded DNA based on the concentration of free glyphosate. The reaction between the antigen-double DNA-gold NPs and immobilized antibody on the substrate was carried out for 2 h. The results of the antigen-antibody reaction were measured on the basis of the fluorescence intensity obtained from comparison with the free antigens at concentrations of 0.01-100 μg mL-1 for the detection of immobilized antigen-double DNA-gold NPs. For the quantitative analysis based on the concentration of glyphosate(0.01-100 μg mL-1), the immunosensor response also revealed the same detection range of glyphosate using DNA detection.

Original languageEnglish
Pages (from-to)12096-12100
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number23
DOIs
Publication statusPublished - 2010 Dec 8

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Keywords

  • antigen-antibody
  • fluorescence
  • glyphosate
  • gold nanoparticles
  • Immunosensor
  • quantitative analysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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