Quantitative detection of glyphosate by simultaneous analysis of UV spectroscopy and fluorescence using DNA-labeled gold nanoparticles

Hee Uk Lee, Hyun Yong Shin, Jin Young Lee, Yoon Seok Song, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A sandwich-type immunosensor composed of antigen-double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement of fluorescence intensity and quantitative analysis of single-stranded DNA based on the concentration of free glyphosate. The reaction between the antigen-double DNA-gold NPs and immobilized antibody on the substrate was carried out for 2 h. The results of the antigen-antibody reaction were measured on the basis of the fluorescence intensity obtained from comparison with the free antigens at concentrations of 0.01-100 μg mL-1 for the detection of immobilized antigen-double DNA-gold NPs. For the quantitative analysis based on the concentration of glyphosate(0.01-100 μg mL-1), the immunosensor response also revealed the same detection range of glyphosate using DNA detection.

Original languageEnglish
Pages (from-to)12096-12100
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number23
DOIs
Publication statusPublished - 2010 Dec 8

Fingerprint

glyphosate
Fluorescence Spectrometry
Ultraviolet spectroscopy
Gold
Nanoparticles
spectroscopy
Fluorescence
fluorescence
antigens
Antigens
Immunosensors
quantitative analysis
DNA
immobilized antigens
antigen-antibody reactions
single-stranded DNA
DNA probes
Antigen-antibody reactions
sandwiches
Antigen-Antibody Reactions

Keywords

  • antigen-antibody
  • fluorescence
  • glyphosate
  • gold nanoparticles
  • Immunosensor
  • quantitative analysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Quantitative detection of glyphosate by simultaneous analysis of UV spectroscopy and fluorescence using DNA-labeled gold nanoparticles. / Lee, Hee Uk; Shin, Hyun Yong; Lee, Jin Young; Song, Yoon Seok; Park, Chulhwan; Kim, Seung Wook.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 23, 08.12.2010, p. 12096-12100.

Research output: Contribution to journalArticle

Lee, Hee Uk ; Shin, Hyun Yong ; Lee, Jin Young ; Song, Yoon Seok ; Park, Chulhwan ; Kim, Seung Wook. / Quantitative detection of glyphosate by simultaneous analysis of UV spectroscopy and fluorescence using DNA-labeled gold nanoparticles. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 23. pp. 12096-12100.
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