Quantitative DIC microscopy using an off-axis self-interference approach

Dan Fu, Seungeun Oh, Wonshik Choi, Toyohiko Yamauchi, August Dorn, Zahid Yaqoob, Ramachandra R. Dasari, Michael S. Feld

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48 Citations (Scopus)

Abstract

Traditional Normarski differential interference contrast (DIC) microscopy is a very powerful method for imaging nonstained biological samples. However, one of its major limitations is the nonquantitative nature of the imaging. To overcome this problem, we developed a quantitative DIC microscopy method based on off-axis sample selfinterference. The digital holography algorithm is applied to obtain quantitative phase gradients in orthogonal directions, which leads to a quantitative phase image through a spiral integration of the phase gradients. This method is practically simple to implement on any standard microscope without stringent requirements on polarization optics. Optical sectioning can be obtained through enlarged illumination NA.

Original languageEnglish
Pages (from-to)2370-2372
Number of pages3
JournalOptics Letters
Volume35
Issue number14
DOIs
Publication statusPublished - 2010 Jul 15

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Fu, D., Oh, S., Choi, W., Yamauchi, T., Dorn, A., Yaqoob, Z., Dasari, R. R., & Feld, M. S. (2010). Quantitative DIC microscopy using an off-axis self-interference approach. Optics Letters, 35(14), 2370-2372. https://doi.org/10.1364/OL.35.002370