Abstract
Refractive index dispersion is an intrinsic optical property and a useful source of contrast in biological imaging studies. In this report, we present the first dispersion phase imaging of living eukaryotic cells. We have developed quantitative dispersion microscopy based on the principle of quantitative phase microscopy. The dual-wavelength quantitative phase microscope makes phase measurements at 310 nm and 400 nm wavelengths to quantify dispersion (refractive index increment ratio) of live cells. The measured dispersion of living HeLa cells is found to be around 1.088, which agrees well with that measured directly for protein solutions using total internal reflection. This technique, together with the dry mass and morphology measurements provided by quantitative phase microscopy, could prove to be a useful tool for distinguishing different types of biomaterials and studying spatial inhomogeneities of biological samples.
| Original language | English |
|---|---|
| Pages (from-to) | 347-353 |
| Number of pages | 7 |
| Journal | Biomedical Optics Express |
| Volume | 1 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 Sep 1 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Biotechnology
Cite this
Quantitative dispersion microscopy. / Fu, Dan; Choi, Wonshik; Sung, Yongjin; Yaqoob, Zahid; Dasari, Ramachandra R.; Feld, Michael.
In: Biomedical Optics Express, Vol. 1, No. 2, 01.09.2010, p. 347-353.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative dispersion microscopy
AU - Fu, Dan
AU - Choi, Wonshik
AU - Sung, Yongjin
AU - Yaqoob, Zahid
AU - Dasari, Ramachandra R.
AU - Feld, Michael
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Refractive index dispersion is an intrinsic optical property and a useful source of contrast in biological imaging studies. In this report, we present the first dispersion phase imaging of living eukaryotic cells. We have developed quantitative dispersion microscopy based on the principle of quantitative phase microscopy. The dual-wavelength quantitative phase microscope makes phase measurements at 310 nm and 400 nm wavelengths to quantify dispersion (refractive index increment ratio) of live cells. The measured dispersion of living HeLa cells is found to be around 1.088, which agrees well with that measured directly for protein solutions using total internal reflection. This technique, together with the dry mass and morphology measurements provided by quantitative phase microscopy, could prove to be a useful tool for distinguishing different types of biomaterials and studying spatial inhomogeneities of biological samples.
AB - Refractive index dispersion is an intrinsic optical property and a useful source of contrast in biological imaging studies. In this report, we present the first dispersion phase imaging of living eukaryotic cells. We have developed quantitative dispersion microscopy based on the principle of quantitative phase microscopy. The dual-wavelength quantitative phase microscope makes phase measurements at 310 nm and 400 nm wavelengths to quantify dispersion (refractive index increment ratio) of live cells. The measured dispersion of living HeLa cells is found to be around 1.088, which agrees well with that measured directly for protein solutions using total internal reflection. This technique, together with the dry mass and morphology measurements provided by quantitative phase microscopy, could prove to be a useful tool for distinguishing different types of biomaterials and studying spatial inhomogeneities of biological samples.
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U2 - 10.1364/BOE.1.000347
DO - 10.1364/BOE.1.000347
M3 - Article
VL - 1
SP - 347
EP - 353
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 2
ER -