Quantitative evaluation of laryngeal function in glottal insufficiency animal model for tissue engineering approach

Seong Keun Kwon, Eun Kyeung Lee, Hee Bok Kim, Jae-Jun Song, Chang Gun Cho, Seok Won Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Injection laryngoplasty is widely used for treatment of vocal fold palsy. To test the efficacy and safety of newly developed injection materials for vocal fold augmentation, we need reliable animal model for objective evaluation of glottal insufficiency. In this study, we aimed to develop an animal model to quantitatively evaluate the histological and functional changes of the paralyzed vocal fold. Left side recurrent laryngeal nerves were identified at tracheoesophageal groove of the New Zealand white rabbits and they were resected. Endoscopic examination was performed immediately after operation and 1, 2, 4, 8, 12 week after the operation to identify left vocal fold immobility. Total laryngectomy was performed 1, 2, 4, 8, 12 weeks after the operation. We have developed the method to quantify the changes of the intralaryngeal muscles. Histologic exam revealed significant decrease in thyroarytenoid muscle volume in the paralyzed side 12 weeks after the operation. We have also developed the method to quantify the laryngeal functions by analyzing vocal fold vibration. High speed recording of induced phonation showed irregular and asymmetric movement of vocal fold in the glottal insufficiency model. Videokymographic analysis showed increased open quotient and asymmetric index compared with the normal vocal fold. This model could be used to provide fundamental changes related with vocal fold paralysis. Even more, this animal model could be utilized to test efficacy and safety of various injection materials to augment the vocal fold.

Original languageEnglish
Pages (from-to)322-328
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Volume10
Issue number6
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

Fingerprint

Vocal Cords
Tissue Engineering
Tissue engineering
Animals
Animal Models
Muscle
Injections
Laryngoplasty
Laryngeal Muscles
Recurrent Laryngeal Nerve
Safety
Phonation
Vocal Cord Paralysis
Laryngectomy
Vibration
Paralysis
Rabbits
Muscles

Keywords

  • animal model
  • glottal insufficiency
  • injection laryngoplasty
  • vocal fold paralysis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Quantitative evaluation of laryngeal function in glottal insufficiency animal model for tissue engineering approach. / Kwon, Seong Keun; Lee, Eun Kyeung; Kim, Hee Bok; Song, Jae-Jun; Cho, Chang Gun; Park, Seok Won.

In: Tissue Engineering and Regenerative Medicine, Vol. 10, No. 6, 01.01.2013, p. 322-328.

Research output: Contribution to journalArticle

Kwon, Seong Keun ; Lee, Eun Kyeung ; Kim, Hee Bok ; Song, Jae-Jun ; Cho, Chang Gun ; Park, Seok Won. / Quantitative evaluation of laryngeal function in glottal insufficiency animal model for tissue engineering approach. In: Tissue Engineering and Regenerative Medicine. 2013 ; Vol. 10, No. 6. pp. 322-328.
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