Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry

Alicja Gasecka, Tsai Jung Han, Cyril Favard, Bong-Rae Cho, Sophie Brasselet

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the investigation of the molecular angular distribution in giant unilamellar vesicles formed by liquid-ordered and liquid-disordered micro-domains, and in COS-7 cell membranes. The highest order contrast between ordered and disordered domains is obtained for dyes locating within the membrane acyl chains.

Original languageEnglish
Pages (from-to)2854-2862
Number of pages9
JournalBiophysical Journal
Volume97
Issue number10
DOIs
Publication statusPublished - 2009 Nov 15

Fingerprint

Unilamellar Liposomes
Fluorescence Polarization
Molecular Imaging
COS Cells
Membrane Lipids
Photons
Microscopy
Coloring Agents
Fluorescence
Cell Membrane
Membranes

ASJC Scopus subject areas

  • Biophysics

Cite this

Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry. / Gasecka, Alicja; Han, Tsai Jung; Favard, Cyril; Cho, Bong-Rae; Brasselet, Sophie.

In: Biophysical Journal, Vol. 97, No. 10, 15.11.2009, p. 2854-2862.

Research output: Contribution to journalArticle

Gasecka, Alicja ; Han, Tsai Jung ; Favard, Cyril ; Cho, Bong-Rae ; Brasselet, Sophie. / Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry. In: Biophysical Journal. 2009 ; Vol. 97, No. 10. pp. 2854-2862.
@article{4ec190ccd04e413d9d201175f2f1e859,
title = "Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry",
abstract = "We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the investigation of the molecular angular distribution in giant unilamellar vesicles formed by liquid-ordered and liquid-disordered micro-domains, and in COS-7 cell membranes. The highest order contrast between ordered and disordered domains is obtained for dyes locating within the membrane acyl chains.",
author = "Alicja Gasecka and Han, {Tsai Jung} and Cyril Favard and Bong-Rae Cho and Sophie Brasselet",
year = "2009",
month = "11",
day = "15",
doi = "10.1016/j.bpj.2009.08.052",
language = "English",
volume = "97",
pages = "2854--2862",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "10",

}

TY - JOUR

T1 - Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry

AU - Gasecka, Alicja

AU - Han, Tsai Jung

AU - Favard, Cyril

AU - Cho, Bong-Rae

AU - Brasselet, Sophie

PY - 2009/11/15

Y1 - 2009/11/15

N2 - We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the investigation of the molecular angular distribution in giant unilamellar vesicles formed by liquid-ordered and liquid-disordered micro-domains, and in COS-7 cell membranes. The highest order contrast between ordered and disordered domains is obtained for dyes locating within the membrane acyl chains.

AB - We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the investigation of the molecular angular distribution in giant unilamellar vesicles formed by liquid-ordered and liquid-disordered micro-domains, and in COS-7 cell membranes. The highest order contrast between ordered and disordered domains is obtained for dyes locating within the membrane acyl chains.

UR - http://www.scopus.com/inward/record.url?scp=72149121153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72149121153&partnerID=8YFLogxK

U2 - 10.1016/j.bpj.2009.08.052

DO - 10.1016/j.bpj.2009.08.052

M3 - Article

VL - 97

SP - 2854

EP - 2862

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 10

ER -