Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi; Yongjin Sung; Yongkeun Park; Christopher Fang-Yen; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi, Yongjin Sung, Yongkeun Park, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

Original language	English
Title of host publication	Optics InfoBase Conference Papers
Publication status	Published - 2009 Dec 1
Externally published	Yes
Event	Novel Techniques in Microscopy, NTM 2009 - Vancouver, Canada Duration: 2009 Apr 26 → 2009 Apr 30

Other

Other	Novel Techniques in Microscopy, NTM 2009
Country	Canada
City	Vancouver
Period	09/4/26 → 09/4/30

ASJC Scopus subject areas

Instrumentation
Atomic and Molecular Physics, and Optics

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Cite this

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title = "Quantitative live cell imaging with tomographic phase microscopy",

abstract = "In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.",

author = "Wonshik Choi and Yongjin Sung and Yongkeun Park and Christopher Fang-Yen and Kamran Badizadegan and Dasari, {Ramachandra R.} and Feld, {Michael S.}",

year = "2009",

month = dec,

day = "1",

language = "English",

isbn = "9781557528711",

booktitle = "Optics InfoBase Conference Papers",

note = "Novel Techniques in Microscopy, NTM 2009 ; Conference date: 26-04-2009 Through 30-04-2009",

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T1 - Quantitative live cell imaging with tomographic phase microscopy

AU - Choi, Wonshik

AU - Sung, Yongjin

AU - Park, Yongkeun

AU - Fang-Yen, Christopher

AU - Badizadegan, Kamran

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

AB - In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

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UR - http://www.scopus.com/inward/citedby.url?scp=84898081384&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84898081384

SN - 9781557528711

BT - Optics InfoBase Conference Papers

T2 - Novel Techniques in Microscopy, NTM 2009

Y2 - 26 April 2009 through 30 April 2009

ER -