Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi, Yongjin Sung, Yongkeun Park, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution


In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

Original languageEnglish
Title of host publicationOptics InfoBase Conference Papers
Publication statusPublished - 2009 Dec 1
Externally publishedYes
EventNovel Techniques in Microscopy, NTM 2009 - Vancouver, Canada
Duration: 2009 Apr 262009 Apr 30


OtherNovel Techniques in Microscopy, NTM 2009

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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    Choi, W., Sung, Y., Park, Y., Fang-Yen, C., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2009). Quantitative live cell imaging with tomographic phase microscopy. In Optics InfoBase Conference Papers