Abstract
In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.
| Original language | English |
|---|---|
| Title of host publication | Optics InfoBase Conference Papers |
| Publication status | Published - 2009 Dec 1 |
| Externally published | Yes |
| Event | Novel Techniques in Microscopy, NTM 2009 - Vancouver, Canada Duration: 2009 Apr 26 → 2009 Apr 30 |
Other
| Other | Novel Techniques in Microscopy, NTM 2009 |
|---|---|
| Country | Canada |
| City | Vancouver |
| Period | 09/4/26 → 09/4/30 |
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ASJC Scopus subject areas
- Instrumentation
- Atomic and Molecular Physics, and Optics
Cite this
Quantitative live cell imaging with tomographic phase microscopy. / Choi, Wonshik; Sung, Yongjin; Park, Yongkeun; Fang-Yen, Christopher; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.
Optics InfoBase Conference Papers. 2009.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Quantitative live cell imaging with tomographic phase microscopy
AU - Choi, Wonshik
AU - Sung, Yongjin
AU - Park, Yongkeun
AU - Fang-Yen, Christopher
AU - Badizadegan, Kamran
AU - Dasari, Ramachandra R.
AU - Feld, Michael S.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.
AB - In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.
UR - http://www.scopus.com/inward/record.url?scp=84898081384&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898081384&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84898081384
SN - 9781557528711
BT - Optics InfoBase Conference Papers
ER -