Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi, Yongjin Sung, Yongkeun Park, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

Original languageEnglish
Title of host publicationOptics InfoBase Conference Papers
Publication statusPublished - 2009 Dec 1
Externally publishedYes
EventNovel Techniques in Microscopy, NTM 2009 - Vancouver, Canada
Duration: 2009 Apr 262009 Apr 30

Other

OtherNovel Techniques in Microscopy, NTM 2009
CountryCanada
CityVancouver
Period09/4/2609/4/30

Fingerprint

phase contrast
Microscopic examination
Microscopes
microscopes
Cytology
Tissue
microscopy
Imaging techniques
Hemoglobin
hemoglobin
erythrocytes
infectious diseases
cells
progressions
Refractive index
Blood
Lighting
illumination
Cells
refractivity

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Choi, W., Sung, Y., Park, Y., Fang-Yen, C., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2009). Quantitative live cell imaging with tomographic phase microscopy. In Optics InfoBase Conference Papers

Quantitative live cell imaging with tomographic phase microscopy. / Choi, Wonshik; Sung, Yongjin; Park, Yongkeun; Fang-Yen, Christopher; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.

Optics InfoBase Conference Papers. 2009.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Choi, W, Sung, Y, Park, Y, Fang-Yen, C, Badizadegan, K, Dasari, RR & Feld, MS 2009, Quantitative live cell imaging with tomographic phase microscopy. in Optics InfoBase Conference Papers. Novel Techniques in Microscopy, NTM 2009, Vancouver, Canada, 09/4/26.
Choi W, Sung Y, Park Y, Fang-Yen C, Badizadegan K, Dasari RR et al. Quantitative live cell imaging with tomographic phase microscopy. In Optics InfoBase Conference Papers. 2009
Choi, Wonshik ; Sung, Yongjin ; Park, Yongkeun ; Fang-Yen, Christopher ; Badizadegan, Kamran ; Dasari, Ramachandra R. ; Feld, Michael S. / Quantitative live cell imaging with tomographic phase microscopy. Optics InfoBase Conference Papers. 2009.
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