Quantitative live cell imaging with tomographic phase microscopy

Wonshik Choi, Yongjin Sung, Yongkeun Park, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In visualizing transparent biological cells and tissues, the phase contrast microscope and its related techniques have been a cornerstone of nearly every cell biology laboratory. However, phase contrast methods are inherently qualitative and lack in 3-D imaging capability. We introduce a novel tomographic microscopy for quantitative three-dimensional mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. With this instrument, we performed noninvasive quantification of the hemoglobin content in the red blood cells as the disease progression of malaria infection.

Original languageEnglish
Title of host publicationNovel Techniques in Microscopy, NTM 2009
PublisherOptical Society of America (OSA)
ISBN (Print)9781557528711
DOIs
Publication statusPublished - 2009
EventNovel Techniques in Microscopy, NTM 2009 - Vancouver, Canada
Duration: 2009 Apr 262009 Apr 30

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Other

OtherNovel Techniques in Microscopy, NTM 2009
CountryCanada
CityVancouver
Period09/4/2609/4/30

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Choi, W., Sung, Y., Park, Y., Fang-Yen, C., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2009). Quantitative live cell imaging with tomographic phase microscopy. In Novel Techniques in Microscopy, NTM 2009 (Optics InfoBase Conference Papers). Optical Society of America (OSA). https://doi.org/10.1364/ntm.2009.ntua1